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Transfection reagent artefact likely accounts for some reports of extracellular vesicle function
Extracellular vesicles (EV) are important mediators of cell communication and physiology. EVs are frequently investigated by transiently transfecting cells with plasmid DNA to produce EVs modified with protein(s) or nucleic acid(s) of interest. DNA‐transfection reagent complexes (DTC) are approximat...
Autores principales: | McConnell, Russell E., Youniss, Madeleine, Gnanasambandam, Bhargavee, Shah, Palak, Zhang, Wei, Finn, Jonathan D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549734/ https://www.ncbi.nlm.nih.gov/pubmed/36214493 http://dx.doi.org/10.1002/jev2.12253 |
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