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Transfection reagent artefact likely accounts for some reports of extracellular vesicle function

Extracellular vesicles (EV) are important mediators of cell communication and physiology. EVs are frequently investigated by transiently transfecting cells with plasmid DNA to produce EVs modified with protein(s) or nucleic acid(s) of interest. DNA‐transfection reagent complexes (DTC) are approximat...

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Detalles Bibliográficos
Autores principales: McConnell, Russell E., Youniss, Madeleine, Gnanasambandam, Bhargavee, Shah, Palak, Zhang, Wei, Finn, Jonathan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549734/
https://www.ncbi.nlm.nih.gov/pubmed/36214493
http://dx.doi.org/10.1002/jev2.12253

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