Cargando…
Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells
There is a substantial need for the development of biomaterials for protecting hematopoietic stem cells and enhancing hematopoiesis after radiation damage. Bacterial exopolysaccharide (EPS) has been shown to be very attractive to researchers as a radioprotectant owing to its high antioxidant, anti-c...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549790/ https://www.ncbi.nlm.nih.gov/pubmed/36226052 http://dx.doi.org/10.3389/fonc.2022.898185 |
_version_ | 1784805748985823232 |
---|---|
author | Park, Hae Ran Lee, Ji Hee Ji, Hyun Jung Lim, Sangyong Ahn, Ki Bum Seo, Ho Seong |
author_facet | Park, Hae Ran Lee, Ji Hee Ji, Hyun Jung Lim, Sangyong Ahn, Ki Bum Seo, Ho Seong |
author_sort | Park, Hae Ran |
collection | PubMed |
description | There is a substantial need for the development of biomaterials for protecting hematopoietic stem cells and enhancing hematopoiesis after radiation damage. Bacterial exopolysaccharide (EPS) has been shown to be very attractive to researchers as a radioprotectant owing to its high antioxidant, anti-cancer, and limited adverse effects. In the present study, we isolated EPS from a novel strain, Deinococcus radiodurans BRD125, which produces EPS in high abundance, and investigated its applicability as a radioprotective biomaterial. We found that EPS isolated from EPS-rich D. radiodurans BRD125 (DeinoPol-BRD125) had an excellent free-radical scavenging effect and reduced irradiation-induced apoptosis. In addition, bone-marrow and spleen-cell apoptosis in irradiated mice were significantly reduced by DeinoPol-BRD125 administration. DeinoPol-BRD125 enhanced the expression of hematopoiesis-related cytokines such as GM-CSF, G-GSF, M-CSF, and SCF, thereby enhancing hematopoietic stem cells protection and regeneration. Taken together, our findings are the first to report the immunological mechanism of a novel radioprotectant, DeinoPol-BRD125, which might constitute an ideal radioprotective and radiation mitigating agent as a supplement drug during radiotherapy. |
format | Online Article Text |
id | pubmed-9549790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95497902022-10-11 Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells Park, Hae Ran Lee, Ji Hee Ji, Hyun Jung Lim, Sangyong Ahn, Ki Bum Seo, Ho Seong Front Oncol Oncology There is a substantial need for the development of biomaterials for protecting hematopoietic stem cells and enhancing hematopoiesis after radiation damage. Bacterial exopolysaccharide (EPS) has been shown to be very attractive to researchers as a radioprotectant owing to its high antioxidant, anti-cancer, and limited adverse effects. In the present study, we isolated EPS from a novel strain, Deinococcus radiodurans BRD125, which produces EPS in high abundance, and investigated its applicability as a radioprotective biomaterial. We found that EPS isolated from EPS-rich D. radiodurans BRD125 (DeinoPol-BRD125) had an excellent free-radical scavenging effect and reduced irradiation-induced apoptosis. In addition, bone-marrow and spleen-cell apoptosis in irradiated mice were significantly reduced by DeinoPol-BRD125 administration. DeinoPol-BRD125 enhanced the expression of hematopoiesis-related cytokines such as GM-CSF, G-GSF, M-CSF, and SCF, thereby enhancing hematopoietic stem cells protection and regeneration. Taken together, our findings are the first to report the immunological mechanism of a novel radioprotectant, DeinoPol-BRD125, which might constitute an ideal radioprotective and radiation mitigating agent as a supplement drug during radiotherapy. Frontiers Media S.A. 2022-09-26 /pmc/articles/PMC9549790/ /pubmed/36226052 http://dx.doi.org/10.3389/fonc.2022.898185 Text en Copyright © 2022 Park, Lee, Ji, Lim, Ahn and Seo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Park, Hae Ran Lee, Ji Hee Ji, Hyun Jung Lim, Sangyong Ahn, Ki Bum Seo, Ho Seong Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells |
title | Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells |
title_full | Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells |
title_fullStr | Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells |
title_full_unstemmed | Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells |
title_short | Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells |
title_sort | radioprotection of deinococcal exopolysaccharide brd125 by regenerating hematopoietic stem cells |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549790/ https://www.ncbi.nlm.nih.gov/pubmed/36226052 http://dx.doi.org/10.3389/fonc.2022.898185 |
work_keys_str_mv | AT parkhaeran radioprotectionofdeinococcalexopolysaccharidebrd125byregeneratinghematopoieticstemcells AT leejihee radioprotectionofdeinococcalexopolysaccharidebrd125byregeneratinghematopoieticstemcells AT jihyunjung radioprotectionofdeinococcalexopolysaccharidebrd125byregeneratinghematopoieticstemcells AT limsangyong radioprotectionofdeinococcalexopolysaccharidebrd125byregeneratinghematopoieticstemcells AT ahnkibum radioprotectionofdeinococcalexopolysaccharidebrd125byregeneratinghematopoieticstemcells AT seohoseong radioprotectionofdeinococcalexopolysaccharidebrd125byregeneratinghematopoieticstemcells |