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Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study
BACKGROUND: SARS-CoV-2 pre-existing T-cell immune reactivity can be present in some people. A general perturbation of the main peripheral lymphocyte subsets has been described in severe COVID-19 patients, but very few studies assessed the general memory T-cell homeostasis in the acute phase of COVID...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549841/ https://www.ncbi.nlm.nih.gov/pubmed/36217141 http://dx.doi.org/10.1186/s12931-022-02190-8 |
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author | Bekbossynova, Makhabbat Akhmaltdinova, Lyudmila Dossybayeva, Kuanysh Tauekelova, Ainur Smagulova, Zauresh Tsechoeva, Tatyana Turebayeva, Gulsimzhan Sailybayeva, Aliya Kalila, Zhanar Mirashirova, Tahmina Muratov, Timur Poddighe, Dimitri |
author_facet | Bekbossynova, Makhabbat Akhmaltdinova, Lyudmila Dossybayeva, Kuanysh Tauekelova, Ainur Smagulova, Zauresh Tsechoeva, Tatyana Turebayeva, Gulsimzhan Sailybayeva, Aliya Kalila, Zhanar Mirashirova, Tahmina Muratov, Timur Poddighe, Dimitri |
author_sort | Bekbossynova, Makhabbat |
collection | PubMed |
description | BACKGROUND: SARS-CoV-2 pre-existing T-cell immune reactivity can be present in some people. A general perturbation of the main peripheral lymphocyte subsets has been described in severe COVID-19 patients, but very few studies assessed the general memory T-cell homeostasis in the acute phase of COVID-19. Here, we performed a general analysis of the main memory T cell populations in the peripheral blood of patients admitted to the hospital for a confirmed or probable COVID-19 diagnosis. METHODS: In this cross-sectional study, adult patients (aged ≥ 18 years) needing hospital admission for respiratory disease due to confirmed or probable COVID-19, were recruited before starting the therapeutic protocol for this disease. In addition to the assessment of the general lymphocyte subpopulations in the early phase of COVID-19, central memory T cells (Tm(centr) cells: CD45RO+CCR7+) and effector memory T cells (Tm(eff) cells: CD45RO+CCR7−) were assessed by multi-color flow cytometry, in comparison to a control group. RESULTS: During the study period, 148 study participants were recruited. Among them, 58 patients turned out positive for SARS-CoV-2 PCR (including both patients with interstitial pneumonia [PCR+Pn+] and without this complication [PCR+Pn−]), whereas the remaining 90 patients resulted to be SARS-CoV-2 PCR negative, even though all were affected with interstitial pneumonia [PCR−Pn+]. Additionally, 28 control patients without any ongoing respiratory disease were recruited. A clear unbalance in the T memory compartment emerged from this analysis on the whole pool of T cells (CD3+ cells), showing a significant increase in Tm(centr) cells and, conversely, a significant decrease in Tm(eff) cells in both pneumonia groups (PCR+Pn+ and PCR−Pn+) compared to the controls; PCR+Pn− group showed trends comprised between patients with pneumonia (from one side) and the control group (from the other side). This perturbation inside the memory T cell compartment was also observed in the individual analysis of the four main T cell subpopulations, based upon the differential expression of CD4 and/or CD8 markers. CONCLUSION: Overall, we observed both absolute and relative increases of Tm(centr) cells and decrease of Tm(eff) cells in patients affected with interstitial pneumonia (regardless of the positive or negative results of SARS-CoV-2 PCR), compared to controls. These results need confirmation from additional research, in order to consider this finding as a potential biological marker of interstitial lung involvement in patients affected with viral respiratory infections. |
format | Online Article Text |
id | pubmed-9549841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-95498412022-10-11 Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study Bekbossynova, Makhabbat Akhmaltdinova, Lyudmila Dossybayeva, Kuanysh Tauekelova, Ainur Smagulova, Zauresh Tsechoeva, Tatyana Turebayeva, Gulsimzhan Sailybayeva, Aliya Kalila, Zhanar Mirashirova, Tahmina Muratov, Timur Poddighe, Dimitri Respir Res Correspondence BACKGROUND: SARS-CoV-2 pre-existing T-cell immune reactivity can be present in some people. A general perturbation of the main peripheral lymphocyte subsets has been described in severe COVID-19 patients, but very few studies assessed the general memory T-cell homeostasis in the acute phase of COVID-19. Here, we performed a general analysis of the main memory T cell populations in the peripheral blood of patients admitted to the hospital for a confirmed or probable COVID-19 diagnosis. METHODS: In this cross-sectional study, adult patients (aged ≥ 18 years) needing hospital admission for respiratory disease due to confirmed or probable COVID-19, were recruited before starting the therapeutic protocol for this disease. In addition to the assessment of the general lymphocyte subpopulations in the early phase of COVID-19, central memory T cells (Tm(centr) cells: CD45RO+CCR7+) and effector memory T cells (Tm(eff) cells: CD45RO+CCR7−) were assessed by multi-color flow cytometry, in comparison to a control group. RESULTS: During the study period, 148 study participants were recruited. Among them, 58 patients turned out positive for SARS-CoV-2 PCR (including both patients with interstitial pneumonia [PCR+Pn+] and without this complication [PCR+Pn−]), whereas the remaining 90 patients resulted to be SARS-CoV-2 PCR negative, even though all were affected with interstitial pneumonia [PCR−Pn+]. Additionally, 28 control patients without any ongoing respiratory disease were recruited. A clear unbalance in the T memory compartment emerged from this analysis on the whole pool of T cells (CD3+ cells), showing a significant increase in Tm(centr) cells and, conversely, a significant decrease in Tm(eff) cells in both pneumonia groups (PCR+Pn+ and PCR−Pn+) compared to the controls; PCR+Pn− group showed trends comprised between patients with pneumonia (from one side) and the control group (from the other side). This perturbation inside the memory T cell compartment was also observed in the individual analysis of the four main T cell subpopulations, based upon the differential expression of CD4 and/or CD8 markers. CONCLUSION: Overall, we observed both absolute and relative increases of Tm(centr) cells and decrease of Tm(eff) cells in patients affected with interstitial pneumonia (regardless of the positive or negative results of SARS-CoV-2 PCR), compared to controls. These results need confirmation from additional research, in order to consider this finding as a potential biological marker of interstitial lung involvement in patients affected with viral respiratory infections. BioMed Central 2022-10-10 2022 /pmc/articles/PMC9549841/ /pubmed/36217141 http://dx.doi.org/10.1186/s12931-022-02190-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Correspondence Bekbossynova, Makhabbat Akhmaltdinova, Lyudmila Dossybayeva, Kuanysh Tauekelova, Ainur Smagulova, Zauresh Tsechoeva, Tatyana Turebayeva, Gulsimzhan Sailybayeva, Aliya Kalila, Zhanar Mirashirova, Tahmina Muratov, Timur Poddighe, Dimitri Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study |
title | Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study |
title_full | Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study |
title_fullStr | Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study |
title_full_unstemmed | Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study |
title_short | Central and effector memory T cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a COVID-19 study |
title_sort | central and effector memory t cells in peripheral blood of patients with interstitial pneumonia: preliminary clues from a covid-19 study |
topic | Correspondence |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549841/ https://www.ncbi.nlm.nih.gov/pubmed/36217141 http://dx.doi.org/10.1186/s12931-022-02190-8 |
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