Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil

BACKGROUND: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODS: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some...

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Detalles Bibliográficos
Autores principales: Coletti, Thaís de Moura, Romano, Camila Malta, Urbinatti, Paulo Roberto, Teixeira, Renildo Souza, Pedrosa, Leila Weiss de Almeida, Nardi, Marcello Schiavo, Natal, Delsio, da Costa, Antônio Charlys, de Jesus, Jaqueline Goes, Claro, Ingra Morales, Sabino, Ester Cerdeira, Witkin, Steven S., Marrelli, Mauro Toledo, Fernandes, Licia Natal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549946/
https://www.ncbi.nlm.nih.gov/pubmed/36169488
http://dx.doi.org/10.1590/0037-8682-0067-2022
Descripción
Sumario:BACKGROUND: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODS: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. RESULTS: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). CONCLUSIONS: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.

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