Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil

BACKGROUND: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODS: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some...

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Autores principales: Coletti, Thaís de Moura, Romano, Camila Malta, Urbinatti, Paulo Roberto, Teixeira, Renildo Souza, Pedrosa, Leila Weiss de Almeida, Nardi, Marcello Schiavo, Natal, Delsio, da Costa, Antônio Charlys, de Jesus, Jaqueline Goes, Claro, Ingra Morales, Sabino, Ester Cerdeira, Witkin, Steven S., Marrelli, Mauro Toledo, Fernandes, Licia Natal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549946/
https://www.ncbi.nlm.nih.gov/pubmed/36169488
http://dx.doi.org/10.1590/0037-8682-0067-2022
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author Coletti, Thaís de Moura
Romano, Camila Malta
Urbinatti, Paulo Roberto
Teixeira, Renildo Souza
Pedrosa, Leila Weiss de Almeida
Nardi, Marcello Schiavo
Natal, Delsio
da Costa, Antônio Charlys
de Jesus, Jaqueline Goes
Claro, Ingra Morales
Sabino, Ester Cerdeira
Witkin, Steven S.
Marrelli, Mauro Toledo
Fernandes, Licia Natal
author_facet Coletti, Thaís de Moura
Romano, Camila Malta
Urbinatti, Paulo Roberto
Teixeira, Renildo Souza
Pedrosa, Leila Weiss de Almeida
Nardi, Marcello Schiavo
Natal, Delsio
da Costa, Antônio Charlys
de Jesus, Jaqueline Goes
Claro, Ingra Morales
Sabino, Ester Cerdeira
Witkin, Steven S.
Marrelli, Mauro Toledo
Fernandes, Licia Natal
author_sort Coletti, Thaís de Moura
collection PubMed
description BACKGROUND: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODS: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. RESULTS: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). CONCLUSIONS: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence.
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spelling pubmed-95499462022-10-25 Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil Coletti, Thaís de Moura Romano, Camila Malta Urbinatti, Paulo Roberto Teixeira, Renildo Souza Pedrosa, Leila Weiss de Almeida Nardi, Marcello Schiavo Natal, Delsio da Costa, Antônio Charlys de Jesus, Jaqueline Goes Claro, Ingra Morales Sabino, Ester Cerdeira Witkin, Steven S. Marrelli, Mauro Toledo Fernandes, Licia Natal Rev Soc Bras Med Trop Short Communication BACKGROUND: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. METHODS: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. RESULTS: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). CONCLUSIONS: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence. Sociedade Brasileira de Medicina Tropical - SBMT 2022-09-26 /pmc/articles/PMC9549946/ /pubmed/36169488 http://dx.doi.org/10.1590/0037-8682-0067-2022 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Coletti, Thaís de Moura
Romano, Camila Malta
Urbinatti, Paulo Roberto
Teixeira, Renildo Souza
Pedrosa, Leila Weiss de Almeida
Nardi, Marcello Schiavo
Natal, Delsio
da Costa, Antônio Charlys
de Jesus, Jaqueline Goes
Claro, Ingra Morales
Sabino, Ester Cerdeira
Witkin, Steven S.
Marrelli, Mauro Toledo
Fernandes, Licia Natal
Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
title Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
title_full Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
title_fullStr Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
title_full_unstemmed Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
title_short Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil
title_sort characterization of insect-specific culex flavivirus (flaviviridae) nucleotide sequences in mosquitoes from urban parks in são paulo, brazil
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549946/
https://www.ncbi.nlm.nih.gov/pubmed/36169488
http://dx.doi.org/10.1590/0037-8682-0067-2022
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