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Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna
It is well-documented that perturbation of the gut bacterial community can influence the reproductive rates of the host. Less is known about how natural ecological processes can change the bacterial composition in the gut and how such changes influence the reproductive rate of the host. Here, we pro...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550005/ https://www.ncbi.nlm.nih.gov/pubmed/36073495 http://dx.doi.org/10.1093/femsec/fiac105 |
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author | Obrestad, Kathrine Einum, Sigurd Vadstein, Olav |
author_facet | Obrestad, Kathrine Einum, Sigurd Vadstein, Olav |
author_sort | Obrestad, Kathrine |
collection | PubMed |
description | It is well-documented that perturbation of the gut bacterial community can influence the reproductive rates of the host. Less is known about how natural ecological processes can change the bacterial composition in the gut and how such changes influence the reproductive rate of the host. Here, we provide novel experimental insights into such processes using the clonally reproducing water flea, Daphnia magna. A total of 20 replicate cultures were reared for 5 weeks (Phase 1) to allow for divergence of bacterial communities through stochastic processes (i.e. drift, founder effects, and/or colonization). Duplicate cultures created from each of these were reared for 21 days (Phase 2) while recording reproductive rates. There was a significant repeatability in reproductive rates between these duplicates, suggesting that divergence of the bacterial communities during Phase 1 translated into reproductive rate effects during Phase 2. This was further supported by significant differences in the relative abundance of gut bacteria (investigated by amplicon sequencing of a part of the 16S rRNA gene) between cultures with high and low reproductive rate in Phase 2. These results are consistent with the hypothesis that stochastic processes can cause natural variation in the bacterial composition in the gut, which in turn affect host reproductive rates. |
format | Online Article Text |
id | pubmed-9550005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-95500052022-10-11 Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna Obrestad, Kathrine Einum, Sigurd Vadstein, Olav FEMS Microbiol Ecol Research Article It is well-documented that perturbation of the gut bacterial community can influence the reproductive rates of the host. Less is known about how natural ecological processes can change the bacterial composition in the gut and how such changes influence the reproductive rate of the host. Here, we provide novel experimental insights into such processes using the clonally reproducing water flea, Daphnia magna. A total of 20 replicate cultures were reared for 5 weeks (Phase 1) to allow for divergence of bacterial communities through stochastic processes (i.e. drift, founder effects, and/or colonization). Duplicate cultures created from each of these were reared for 21 days (Phase 2) while recording reproductive rates. There was a significant repeatability in reproductive rates between these duplicates, suggesting that divergence of the bacterial communities during Phase 1 translated into reproductive rate effects during Phase 2. This was further supported by significant differences in the relative abundance of gut bacteria (investigated by amplicon sequencing of a part of the 16S rRNA gene) between cultures with high and low reproductive rate in Phase 2. These results are consistent with the hypothesis that stochastic processes can cause natural variation in the bacterial composition in the gut, which in turn affect host reproductive rates. Oxford University Press 2022-09-07 /pmc/articles/PMC9550005/ /pubmed/36073495 http://dx.doi.org/10.1093/femsec/fiac105 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Obrestad, Kathrine Einum, Sigurd Vadstein, Olav Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna |
title | Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna |
title_full | Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna |
title_fullStr | Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna |
title_full_unstemmed | Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna |
title_short | Stochastic variation in gut bacterial community affects reproductive rates in the water flea Daphnia magna |
title_sort | stochastic variation in gut bacterial community affects reproductive rates in the water flea daphnia magna |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550005/ https://www.ncbi.nlm.nih.gov/pubmed/36073495 http://dx.doi.org/10.1093/femsec/fiac105 |
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