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In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts

Allograft bone tissue has a long history of use. There are two main ways of preserving allografts: by cold (freezing), or at room temperature after an additional cleaning treatment using chemicals. These chemicals are considered potentially harmful to humans. The aim of the study was (i) to assess t...

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Autores principales: Villatte, Guillaume, Erivan, Roger, Descamps, Stéphane, Arque, Pierre, Boisgard, Stéphane, Wittrant, Yohann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550034/
https://www.ncbi.nlm.nih.gov/pubmed/36215295
http://dx.doi.org/10.1371/journal.pone.0275480
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author Villatte, Guillaume
Erivan, Roger
Descamps, Stéphane
Arque, Pierre
Boisgard, Stéphane
Wittrant, Yohann
author_facet Villatte, Guillaume
Erivan, Roger
Descamps, Stéphane
Arque, Pierre
Boisgard, Stéphane
Wittrant, Yohann
author_sort Villatte, Guillaume
collection PubMed
description Allograft bone tissue has a long history of use. There are two main ways of preserving allografts: by cold (freezing), or at room temperature after an additional cleaning treatment using chemicals. These chemicals are considered potentially harmful to humans. The aim of the study was (i) to assess the presence of chemical residues on processed bone allografts and (ii) to compare the in vitro biocompatibility of such allografts with that of frozen allografts. The presence of chemical residues on industrially chemically treated bone was assessed by high performance liquid chromatography (HPLC) after extraction. Biocompatibility analysis was performed on primary osteoblast cultures from Wistar rats grown on bone disks, either frozen (F-bone group) or treated with supercritical carbon dioxide with no added chemical (scCO2-bone group) or industrially treated with chemicals (CT-bone group). Cell viability (XTT) was measured after one week of culture. Osteoblastic differentiation was assessed after 1, 7 and 14 days of culture by measuring alkaline phosphatase (ALP) activity directly on the bone discs and indirectly on the cell mat in the vicinity of the bone discs. Residues of all the chemicals used were found in the CT-bone group. There was no significant difference in cell viability between the three bone groups. Direct and indirect ALP activities were significantly lower (−40% to −80%) in the CT-bone group after 7 and 14 days of culture (p < 0.05). Residues of chemical substances used in the cleaning of bone allografts cause an in vitro decrease in their biocompatibility. Tissue cleaning processes must be developed that limit or replace these chemicals to favor biocompatibility.
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spelling pubmed-95500342022-10-11 In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts Villatte, Guillaume Erivan, Roger Descamps, Stéphane Arque, Pierre Boisgard, Stéphane Wittrant, Yohann PLoS One Research Article Allograft bone tissue has a long history of use. There are two main ways of preserving allografts: by cold (freezing), or at room temperature after an additional cleaning treatment using chemicals. These chemicals are considered potentially harmful to humans. The aim of the study was (i) to assess the presence of chemical residues on processed bone allografts and (ii) to compare the in vitro biocompatibility of such allografts with that of frozen allografts. The presence of chemical residues on industrially chemically treated bone was assessed by high performance liquid chromatography (HPLC) after extraction. Biocompatibility analysis was performed on primary osteoblast cultures from Wistar rats grown on bone disks, either frozen (F-bone group) or treated with supercritical carbon dioxide with no added chemical (scCO2-bone group) or industrially treated with chemicals (CT-bone group). Cell viability (XTT) was measured after one week of culture. Osteoblastic differentiation was assessed after 1, 7 and 14 days of culture by measuring alkaline phosphatase (ALP) activity directly on the bone discs and indirectly on the cell mat in the vicinity of the bone discs. Residues of all the chemicals used were found in the CT-bone group. There was no significant difference in cell viability between the three bone groups. Direct and indirect ALP activities were significantly lower (−40% to −80%) in the CT-bone group after 7 and 14 days of culture (p < 0.05). Residues of chemical substances used in the cleaning of bone allografts cause an in vitro decrease in their biocompatibility. Tissue cleaning processes must be developed that limit or replace these chemicals to favor biocompatibility. Public Library of Science 2022-10-10 /pmc/articles/PMC9550034/ /pubmed/36215295 http://dx.doi.org/10.1371/journal.pone.0275480 Text en © 2022 Villatte et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Villatte, Guillaume
Erivan, Roger
Descamps, Stéphane
Arque, Pierre
Boisgard, Stéphane
Wittrant, Yohann
In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
title In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
title_full In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
title_fullStr In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
title_full_unstemmed In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
title_short In vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
title_sort in vitro osteoblast activity is decreased by residues of chemicals used in the cleaning and viral inactivation process of bone allografts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550034/
https://www.ncbi.nlm.nih.gov/pubmed/36215295
http://dx.doi.org/10.1371/journal.pone.0275480
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