Cargando…
Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ
Tendinopathy is a common tendon disorder characterized by pain, swelling, and dysfunction. Current evidence has demonstrated that the depletion of stem cell pool and non-tenogenic differentiation of tendon-derived stem/progenitor cells (TSPCs) might account for the pathogenesis of tendinopathy. FNDC...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550707/ https://www.ncbi.nlm.nih.gov/pubmed/36125694 http://dx.doi.org/10.1007/s11626-022-00699-2 |
_version_ | 1784805940182122496 |
---|---|
author | Xu, Langhai Chen, Zhonggai Geng, Tingting Ru, Bin Wan, Quan Zhang, Jianbin Li, Shun Cai, Wenjun |
author_facet | Xu, Langhai Chen, Zhonggai Geng, Tingting Ru, Bin Wan, Quan Zhang, Jianbin Li, Shun Cai, Wenjun |
author_sort | Xu, Langhai |
collection | PubMed |
description | Tendinopathy is a common tendon disorder characterized by pain, swelling, and dysfunction. Current evidence has demonstrated that the depletion of stem cell pool and non-tenogenic differentiation of tendon-derived stem/progenitor cells (TSPCs) might account for the pathogenesis of tendinopathy. FNDC5/Irisin, as a novel exercise-induced myokine, is proved to be involved in the exercise-induced protective effects on musculoskeletal disorders. However, whether irisin can affect TSPCs fate is still unknown. To ascertain the roles of irisin on the proliferation and tenogenic differentiation of TSPCs, rat TSPCs were isolated and incubated with irisin. Cell viability, phenotypic changes, and related signaling pathways were evaluated by CCK-8 assay, colony formation assay, real-time PCR, Western blot, immunofluorescence, and proteasome activity assay. We found that irisin treatment increased the proliferative and colony-forming abilities, and promoted the tenogenic differentiation of TSPCs by upregulating the expression of YAP/TAZ. In conclusion, our work showed for the first time that irisin promotes the proliferation and tenogenic differentiation of rat TSPCs in vitro by activating YAP/TAZ, and the process was associated with a ubiquitin-proteasome proteolytic pathway. In conclusion, irisin and agents targeting YAP/TAZ may be promising therapeutic options for tendinopathy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11626-022-00699-2. |
format | Online Article Text |
id | pubmed-9550707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-95507072022-10-12 Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ Xu, Langhai Chen, Zhonggai Geng, Tingting Ru, Bin Wan, Quan Zhang, Jianbin Li, Shun Cai, Wenjun In Vitro Cell Dev Biol Anim Article Tendinopathy is a common tendon disorder characterized by pain, swelling, and dysfunction. Current evidence has demonstrated that the depletion of stem cell pool and non-tenogenic differentiation of tendon-derived stem/progenitor cells (TSPCs) might account for the pathogenesis of tendinopathy. FNDC5/Irisin, as a novel exercise-induced myokine, is proved to be involved in the exercise-induced protective effects on musculoskeletal disorders. However, whether irisin can affect TSPCs fate is still unknown. To ascertain the roles of irisin on the proliferation and tenogenic differentiation of TSPCs, rat TSPCs were isolated and incubated with irisin. Cell viability, phenotypic changes, and related signaling pathways were evaluated by CCK-8 assay, colony formation assay, real-time PCR, Western blot, immunofluorescence, and proteasome activity assay. We found that irisin treatment increased the proliferative and colony-forming abilities, and promoted the tenogenic differentiation of TSPCs by upregulating the expression of YAP/TAZ. In conclusion, our work showed for the first time that irisin promotes the proliferation and tenogenic differentiation of rat TSPCs in vitro by activating YAP/TAZ, and the process was associated with a ubiquitin-proteasome proteolytic pathway. In conclusion, irisin and agents targeting YAP/TAZ may be promising therapeutic options for tendinopathy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11626-022-00699-2. Springer US 2022-09-20 2022 /pmc/articles/PMC9550707/ /pubmed/36125694 http://dx.doi.org/10.1007/s11626-022-00699-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Xu, Langhai Chen, Zhonggai Geng, Tingting Ru, Bin Wan, Quan Zhang, Jianbin Li, Shun Cai, Wenjun Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ |
title | Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ |
title_full | Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ |
title_fullStr | Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ |
title_full_unstemmed | Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ |
title_short | Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ |
title_sort | irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating yap/taz |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550707/ https://www.ncbi.nlm.nih.gov/pubmed/36125694 http://dx.doi.org/10.1007/s11626-022-00699-2 |
work_keys_str_mv | AT xulanghai irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT chenzhonggai irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT gengtingting irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT rubin irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT wanquan irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT zhangjianbin irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT lishun irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz AT caiwenjun irisinpromotestheproliferationandtenogenicdifferentiationofrattendonderivedstemprogenitorcellsviaactivatingyaptaz |