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Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures

The combined effect of surface topography and substrate rigidity in stem cell cultures is still under‐investigated, especially when biodegradable polymers are used. Herein, we assessed human bone marrow stem cell response on aliphatic polyester substrates as a function of anisotropic grooved topogra...

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Autores principales: Ribeiro, Sofia, Pugliese, Eugenia, Korntner, Stefanie H., Fernandes, Emanuel M., Gomes, Manuela E., Reis, Rui L., O'Riordan, Alan, Bayon, Yves, Zeugolis, Dimitrios I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550738/
https://www.ncbi.nlm.nih.gov/pubmed/36247829
http://dx.doi.org/10.1002/elsc.202200029
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author Ribeiro, Sofia
Pugliese, Eugenia
Korntner, Stefanie H.
Fernandes, Emanuel M.
Gomes, Manuela E.
Reis, Rui L.
O'Riordan, Alan
Bayon, Yves
Zeugolis, Dimitrios I.
author_facet Ribeiro, Sofia
Pugliese, Eugenia
Korntner, Stefanie H.
Fernandes, Emanuel M.
Gomes, Manuela E.
Reis, Rui L.
O'Riordan, Alan
Bayon, Yves
Zeugolis, Dimitrios I.
author_sort Ribeiro, Sofia
collection PubMed
description The combined effect of surface topography and substrate rigidity in stem cell cultures is still under‐investigated, especially when biodegradable polymers are used. Herein, we assessed human bone marrow stem cell response on aliphatic polyester substrates as a function of anisotropic grooved topography and rigidity (7 and 12 kPa). Planar tissue culture plastic (TCP, 3 GPa) and aliphatic polyester substrates were used as controls. Cell morphology analysis revealed that grooved substrates caused nuclei orientation/alignment in the direction of the grooves. After 21 days in osteogenic and chondrogenic media, the 3 GPa TCP and the grooved 12 kPa substrate induced significantly higher calcium deposition and alkaline phosphatase (ALP) activity and glycosaminoglycan (GAG) deposition, respectively, than the other groups. After 14 days in tenogenic media, the 3 GPa TCP upregulated four and downregulated four genes; the planar 7 kPa substrate upregulated seven genes and downregulated one gene; and the grooved 12 kPa substrate upregulated seven genes and downregulated one gene. After 21 days in adipogenic media, the softest (7 kPa) substrates induced significantly higher oil droplet deposition than the other substrates and the grooved substrate induced significantly higher droplet deposition than the planar. Our data pave the way for more rational design of bioinspired constructs.
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spelling pubmed-95507382022-10-14 Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures Ribeiro, Sofia Pugliese, Eugenia Korntner, Stefanie H. Fernandes, Emanuel M. Gomes, Manuela E. Reis, Rui L. O'Riordan, Alan Bayon, Yves Zeugolis, Dimitrios I. Eng Life Sci Research Articles The combined effect of surface topography and substrate rigidity in stem cell cultures is still under‐investigated, especially when biodegradable polymers are used. Herein, we assessed human bone marrow stem cell response on aliphatic polyester substrates as a function of anisotropic grooved topography and rigidity (7 and 12 kPa). Planar tissue culture plastic (TCP, 3 GPa) and aliphatic polyester substrates were used as controls. Cell morphology analysis revealed that grooved substrates caused nuclei orientation/alignment in the direction of the grooves. After 21 days in osteogenic and chondrogenic media, the 3 GPa TCP and the grooved 12 kPa substrate induced significantly higher calcium deposition and alkaline phosphatase (ALP) activity and glycosaminoglycan (GAG) deposition, respectively, than the other groups. After 14 days in tenogenic media, the 3 GPa TCP upregulated four and downregulated four genes; the planar 7 kPa substrate upregulated seven genes and downregulated one gene; and the grooved 12 kPa substrate upregulated seven genes and downregulated one gene. After 21 days in adipogenic media, the softest (7 kPa) substrates induced significantly higher oil droplet deposition than the other substrates and the grooved substrate induced significantly higher droplet deposition than the planar. Our data pave the way for more rational design of bioinspired constructs. John Wiley and Sons Inc. 2022-09-13 /pmc/articles/PMC9550738/ /pubmed/36247829 http://dx.doi.org/10.1002/elsc.202200029 Text en © 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Ribeiro, Sofia
Pugliese, Eugenia
Korntner, Stefanie H.
Fernandes, Emanuel M.
Gomes, Manuela E.
Reis, Rui L.
O'Riordan, Alan
Bayon, Yves
Zeugolis, Dimitrios I.
Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
title Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
title_full Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
title_fullStr Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
title_full_unstemmed Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
title_short Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
title_sort assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550738/
https://www.ncbi.nlm.nih.gov/pubmed/36247829
http://dx.doi.org/10.1002/elsc.202200029
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