Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the...

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Detalles Bibliográficos
Autores principales: Cho, Heewon, Kwon, Haw-Young, Sharma, Amit, Lee, Sun Hyeok, Liu, Xiao, Miyamoto, Naoki, Kim, Jong-Jin, Im, Sin-Hyeog, Kang, Nam-Young, Chang, Young-Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550770/
https://www.ncbi.nlm.nih.gov/pubmed/36216803
http://dx.doi.org/10.1038/s41467-022-33526-z
Descripción
Sumario:Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

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