Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the...

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Autores principales: Cho, Heewon, Kwon, Haw-Young, Sharma, Amit, Lee, Sun Hyeok, Liu, Xiao, Miyamoto, Naoki, Kim, Jong-Jin, Im, Sin-Hyeog, Kang, Nam-Young, Chang, Young-Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550770/
https://www.ncbi.nlm.nih.gov/pubmed/36216803
http://dx.doi.org/10.1038/s41467-022-33526-z
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author Cho, Heewon
Kwon, Haw-Young
Sharma, Amit
Lee, Sun Hyeok
Liu, Xiao
Miyamoto, Naoki
Kim, Jong-Jin
Im, Sin-Hyeog
Kang, Nam-Young
Chang, Young-Tae
author_facet Cho, Heewon
Kwon, Haw-Young
Sharma, Amit
Lee, Sun Hyeok
Liu, Xiao
Miyamoto, Naoki
Kim, Jong-Jin
Im, Sin-Hyeog
Kang, Nam-Young
Chang, Young-Tae
author_sort Cho, Heewon
collection PubMed
description Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.
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spelling pubmed-95507702022-10-12 Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target Cho, Heewon Kwon, Haw-Young Sharma, Amit Lee, Sun Hyeok Liu, Xiao Miyamoto, Naoki Kim, Jong-Jin Im, Sin-Hyeog Kang, Nam-Young Chang, Young-Tae Nat Commun Article Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model. Nature Publishing Group UK 2022-10-10 /pmc/articles/PMC9550770/ /pubmed/36216803 http://dx.doi.org/10.1038/s41467-022-33526-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cho, Heewon
Kwon, Haw-Young
Sharma, Amit
Lee, Sun Hyeok
Liu, Xiao
Miyamoto, Naoki
Kim, Jong-Jin
Im, Sin-Hyeog
Kang, Nam-Young
Chang, Young-Tae
Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
title Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
title_full Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
title_fullStr Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
title_full_unstemmed Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
title_short Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
title_sort visualizing inflammation with an m1 macrophage selective probe via glut1 as the gating target
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550770/
https://www.ncbi.nlm.nih.gov/pubmed/36216803
http://dx.doi.org/10.1038/s41467-022-33526-z
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