Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single...

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Detalles Bibliográficos
Autores principales: Xu, Xinzhu, Jia, Shu, Xi, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
News & Views
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550861/
https://www.ncbi.nlm.nih.gov/pubmed/36216797
http://dx.doi.org/10.1038/s41377-022-00983-6
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author Xu, Xinzhu
Jia, Shu
Xi, Peng
author_facet Xu, Xinzhu
Jia, Shu
Xi, Peng
author_sort Xu, Xinzhu
collection PubMed
description A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single photon counting, and drift stabilization, the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference, and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX’s, the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.
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spelling pubmed-95508612022-10-12 Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes Xu, Xinzhu Jia, Shu Xi, Peng Light Sci Appl News & Views A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single photon counting, and drift stabilization, the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference, and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX’s, the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications. Nature Publishing Group UK 2022-10-10 /pmc/articles/PMC9550861/ /pubmed/36216797 http://dx.doi.org/10.1038/s41377-022-00983-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle News & Views
Xu, Xinzhu
Jia, Shu
Xi, Peng
Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
title Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
title_full Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
title_fullStr Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
title_full_unstemmed Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
title_short Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes
title_sort raster-scanning donut simplifies minflux and provides alternative implement on other scanning-based microscopes
topic News & Views
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550861/
https://www.ncbi.nlm.nih.gov/pubmed/36216797
http://dx.doi.org/10.1038/s41377-022-00983-6
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