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FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers

Fluorescence in situ hybridization analysis of numerical chromosomal abnormalities in the sperm of Robertsonian translocation der (13;14) (q10;q10) carriers has focused on a limited number of chromosomes mainly on chromosome 13, 18, 21, X, and Y. Here, we aimed to expand the analysis to all chromoso...

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Autores principales: Zhu, Saijuan, Zhu, Yong, Zhang, Feng, Wu, Junping, Chen, Ying, Sun, Yijuan, Fu, Jing, Wu, Jiangnan, Xiao, Min, Zhang, Shuo, Zhou, Jing, Lei, Caixia, Jiang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9551382/
https://www.ncbi.nlm.nih.gov/pubmed/36238152
http://dx.doi.org/10.3389/fgene.2022.1010568
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author Zhu, Saijuan
Zhu, Yong
Zhang, Feng
Wu, Junping
Chen, Ying
Sun, Yijuan
Fu, Jing
Wu, Jiangnan
Xiao, Min
Zhang, Shuo
Zhou, Jing
Lei, Caixia
Jiang, Feng
author_facet Zhu, Saijuan
Zhu, Yong
Zhang, Feng
Wu, Junping
Chen, Ying
Sun, Yijuan
Fu, Jing
Wu, Jiangnan
Xiao, Min
Zhang, Shuo
Zhou, Jing
Lei, Caixia
Jiang, Feng
author_sort Zhu, Saijuan
collection PubMed
description Fluorescence in situ hybridization analysis of numerical chromosomal abnormalities in the sperm of Robertsonian translocation der (13;14) (q10;q10) carriers has focused on a limited number of chromosomes mainly on chromosome 13, 18, 21, X, and Y. Here, we aimed to expand the analysis to all chromosomes by increasing the number of probes analyzed in fluorescence in situ hybridization. The incidence of numerical abnormalities of all chromosomes (1–22, X, and Y) was determined in sperm from 10 carriers of the Robertsonian translocation der(13;14)(q10;q10) and 10 normozoospermic males to fully assess the effect of translocation-derived chromosome on the segregation of all chromosomes during meiosis. Numerical abnormalities of the two translocated chromosomes were frequently detected in the sperm of der (13;14) translocation carriers, with an average frequency of 14.55% ± 6.00% for chromosome 13 and 13.27% ± 4.14% for chromosome 14. Numerical abnormalities of nontranslocated chromosomes, with an average frequency of 1.77% ± 0.62% (range, 1.16%–3.73%), was lower than that of translocated chromosome. However, the cumulative numerical abnormality of the 22 nontranslocated chromosomes was comparable to that of the two translocated chromosomes. Significantly increased numerical abnormalities in der(13;14) translocation carriers compared with those in normozoospermic males indicates the presence of translocation-derived chromosome disturbances, with translocated chromosomes being most affected; nontranslocated chromosomes were also affected, but to a lesser extent due to a mild interchromosomal effect.
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spelling pubmed-95513822022-10-12 FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers Zhu, Saijuan Zhu, Yong Zhang, Feng Wu, Junping Chen, Ying Sun, Yijuan Fu, Jing Wu, Jiangnan Xiao, Min Zhang, Shuo Zhou, Jing Lei, Caixia Jiang, Feng Front Genet Genetics Fluorescence in situ hybridization analysis of numerical chromosomal abnormalities in the sperm of Robertsonian translocation der (13;14) (q10;q10) carriers has focused on a limited number of chromosomes mainly on chromosome 13, 18, 21, X, and Y. Here, we aimed to expand the analysis to all chromosomes by increasing the number of probes analyzed in fluorescence in situ hybridization. The incidence of numerical abnormalities of all chromosomes (1–22, X, and Y) was determined in sperm from 10 carriers of the Robertsonian translocation der(13;14)(q10;q10) and 10 normozoospermic males to fully assess the effect of translocation-derived chromosome on the segregation of all chromosomes during meiosis. Numerical abnormalities of the two translocated chromosomes were frequently detected in the sperm of der (13;14) translocation carriers, with an average frequency of 14.55% ± 6.00% for chromosome 13 and 13.27% ± 4.14% for chromosome 14. Numerical abnormalities of nontranslocated chromosomes, with an average frequency of 1.77% ± 0.62% (range, 1.16%–3.73%), was lower than that of translocated chromosome. However, the cumulative numerical abnormality of the 22 nontranslocated chromosomes was comparable to that of the two translocated chromosomes. Significantly increased numerical abnormalities in der(13;14) translocation carriers compared with those in normozoospermic males indicates the presence of translocation-derived chromosome disturbances, with translocated chromosomes being most affected; nontranslocated chromosomes were also affected, but to a lesser extent due to a mild interchromosomal effect. Frontiers Media S.A. 2022-09-27 /pmc/articles/PMC9551382/ /pubmed/36238152 http://dx.doi.org/10.3389/fgene.2022.1010568 Text en Copyright © 2022 Zhu, Zhu, Zhang, Wu, Chen, Sun, Fu, Wu, Xiao, Zhang, Zhou, Lei and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhu, Saijuan
Zhu, Yong
Zhang, Feng
Wu, Junping
Chen, Ying
Sun, Yijuan
Fu, Jing
Wu, Jiangnan
Xiao, Min
Zhang, Shuo
Zhou, Jing
Lei, Caixia
Jiang, Feng
FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
title FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
title_full FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
title_fullStr FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
title_full_unstemmed FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
title_short FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
title_sort fish analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9551382/
https://www.ncbi.nlm.nih.gov/pubmed/36238152
http://dx.doi.org/10.3389/fgene.2022.1010568
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