Low-cost programable stroboscopic illumination with sub-microsecond pulses for high-throughput microfluidic applications

To visualize fast-moving objects in microfluidic applications, the image acquisition time must be on the order of a microsecond or less. Commercial imaging systems capable of such short exposure times may be too expensive for many research laboratories. We have therefore developed a low-cost stroboscopic illumination for transmitted-light microscopy based on a high-power LED that can be coupled to a standard industrial camera and provides exposure times on the order of 500 ns. The system is designed to be easily mounted on a standard condenser of an inverted microscope. The illumination is controlled by a fast Arduino-compatible Teensy® 4.0 development board, and the illumination parameters can be set from a PC via a graphical user interface written in Python. The system has been successfully used for high-throughput cell phenotyping using deformability cytometry on a Nikon TE2000 microscope, as well as for droplet microfluidic on an old Olympus inverted microscope.