Cargando…

Deep Single-Shot NanoLC-MS Proteome Profiling with a 1500 Bar UHPLC System, Long Fully Porous Columns, and HRAM MS

[Image: see text] This study demonstrates how the latest ultrahigh-performance liquid chromatography (UHPLC) technology can be combined with high-resolution accurate-mass (HRAM) mass spectrometry (MS) and long columns packed with fully porous particles to improve bottom-up proteomics analysis with n...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zheng, Runsheng, Stejskal, Karel, Pynn, Christopher, Mechtler, Karl, Boychenko, Alexander
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9552226/ https://www.ncbi.nlm.nih.gov/pubmed/36068014 http://dx.doi.org/10.1021/acs.jproteome.2c00270

Ejemplares similares

Deep Proteome Profiling with Reduced Carryover Using Superficially Porous Microfabricated nanoLC Columns
por: Stejskal, Karel, et al.
Publicado: (2022)

Ultrasensitive NanoLC-MS of Subnanogram Protein Samples Using Second Generation Micropillar Array LC Technology with Orbitrap Exploris 480 and FAIMS PRO
por: Stejskal, Karel, et al.
Publicado: (2021)

NanoLC-EI-MS: Perspectives in Biochemical Analysis
por: Pereira dos Santos, Natalia Gabrielly, et al.
Publicado: (2023)

Bioinertization of NanoLC/MS/MS Systems by Depleting Metal Ions From the Mobile Phases for Phosphoproteomics
por: Komori, Yumi, et al.
Publicado: (2023)

Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MS(n)
por: Kurz, Simone, et al.
Publicado: (2021)

Region-Specific Cell Membrane N-Glycome of Functional Mouse Brain Areas Revealed by nanoLC-MS Analysis
por: Barboza, Mariana, et al.
Publicado: (2021)

Analytical Characterization of 3-MeO-PCP and 3-MMC in Seized Products and Biosamples: The Role of LC-HRAM-Orbitrap-MS and Solid Deposition GC-FTIR
por: Frison, Giampietro, et al.
Publicado: (2021)

Optimizing oxytocin LC-MS/MS sensitivity by choosing the right column
por: Ackermans, Mariëtte T.
Publicado: (2021)

Identification of Novel α-Synuclein Isoforms in Human Brain Tissue by using an Online NanoLC-ESI-FTICR-MS Method
por: Öhrfelt, Annika, et al.
Publicado: (2011)

Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations
por: Skottvoll, Frøydis Sved, et al.
Publicado: (2018)

Characterisation of a new online nanoLC-CZE-MS platform and application for the glycosylation profiling of alpha-1-acid glycoprotein
por: Stolz, Alexander, et al.
Publicado: (2021)

Identification and Pharmacokinetic Studies on Complanatuside and Its Major Metabolites in Rats by UHPLC-Q-TOF-MS/MS and LC-MS/MS
por: Yao, Yu-Feng, et al.
Publicado: (2018)

Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS
por: Trakarnsanga, Kongtana, et al.
Publicado: (2014)

Robust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC–MS/MS
por: Bian, Yangyang, et al.
Publicado: (2020)

Validation of an UHPLC-MS/MS Method for Simultaneous Analysis of 11 Mycotoxins in Wheat Flour Using Immunoaffinity Column
por: Heidari, Ghasem, et al.
Publicado: (2019)

Small scale affinity purification and high sensitivity reversed phase nanoLC-MS N-glycan characterization of mAbs and fusion proteins
por: Higel, Fabian, et al.
Publicado: (2014)

Dynamic profiling of intact glucosinolates in radish by combining UHPLC-HRMS/MS and UHPLC-QqQ-MS/MS
por: Yan, Chenghuan, et al.
Publicado: (2023)

iTRAQ-Based Quantitative Proteomic Comparison of 2D and 3D Adipocyte Cell Models Co-cultured with Macrophages Using Online 2D-nanoLC-ESI-MS/MS
por: Lee, Sun Young, et al.
Publicado: (2019)

Analysis of Serotonin in Human Feces Using Solid Phase Extraction and Column-Switching LC-MS/MS
por: Hirabayashi, Yukiko, et al.
Publicado: (2020)

MASPECTRAS: a platform for management and analysis of proteomics LC-MS/MS data
por: Hartler, Jürgen, et al.
Publicado: (2007)

Pharmacokinetics and Metabolism Study of Deep-Sea-Derived Butyrolactone I in Rats by UHPLC–MS/MS and UHPLC–Q-TOF-MS
por: Wu, Liang, et al.
Publicado: (2021)

Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS
por: Ion, Valentin, et al.
Publicado: (2019)

Determination of Paraquat in Arabidopsis Tissues and Protoplasts by UHPLC-MS/MS
por: Zhao, Mingming, et al.
Publicado: (2023)

Comparison of Two Methods, UHPLC-UV and UHPLC-MS/MS, for the Quantification of Polyphenols in Cider Apple Juices
por: Verdu, Cindy F., et al.
Publicado: (2013)

Determination of Phthalate Metabolites in Human Serum and Urine as Biomarkers for Phthalate Exposure Using Column-Switching LC-MS/MS
por: Jeong, Jee Yeon, et al.
Publicado: (2011)

LC, MS(n) and LC–MS/MS studies for the characterization of degradation products of amlodipine
por: Tiwari, Ravi N., et al.
Publicado: (2015)

A Fully Automated Online SPE-LC-MS/MS Method for the Determination of 10 Pharmaceuticals in Wastewater Samples
por: Belay, Masho Hilawie, et al.
Publicado: (2022)

Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS
por: Sim, Hee-Jung, et al.
Publicado: (2013)

Pharmacokinetics and Metabolism Research of Shenkang Injection in Rats Based on UHPLC-MS/MS and UHPLC-Q-Orbitrap HRMS
por: Jiang, Xiaofang, et al.
Publicado: (2020)

Determination of lesinurad in rat plasma by a UHPLC–MS/MS assay
por: Zhou, Xiao-Yang, et al.
Publicado: (2017)

Grape Lipidomics: An Extensive Profiling thorough UHPLC-MS/MS Method
por: Masuero, Domenico, et al.
Publicado: (2021)

Characterization of URB Series Synthetic Cannabinoids by HRMS and UHPLC–MS/MS
por: Agostini, Marco, et al.
Publicado: (2023)

Sensitive Detection of α-Conotoxin GI in Human Plasma Using a Solid-Phase Extraction Column and LC-MS/MS
por: Yu, Shuo, et al.
Publicado: (2017)

Fecal metabolite analysis of Japanese macaques in Yakushima by LC-MS/MS and LC-QTOF-MS
por: TSUCHIDA, Sayaka, et al.
Publicado: (2021)

Identification for metabolism profiles and pharmacokinetic studies of tradition Chinese prescription Ji-Ming-San and its major metabolites in rats by UHPLC-Q-TOF-MS/MS and UHPLC-MS/MS
por: Hsieh, Cheng-Yang, et al.
Publicado: (2023)

Quantification of Paeoniflorin by Fully Validated LC–MS/MS Method: Its Application to Pharmacokinetic Interaction between Paeoniflorin and Verapamil
por: Bao, Bin, et al.
Publicado: (2022)

Largazole Pharmacokinetics in Rats by LC-MS/MS
por: Yu, Mingming, et al.
Publicado: (2014)

Application of LC–MS/MS in the Mycotoxins Studies
por: Gámiz-Gracia, Laura, et al.
Publicado: (2020)

Analysis of isoquinoline alkaloids from Mahonia leschenaultia and Mahonia napaulensis roots using UHPLC-Orbitrap-MS(n) and UHPLC-QqQ(LIT)-MS/MS
por: Singh, Awantika, et al.
Publicado: (2017)

Quantitative determination of vitamin D metabolites in plasma using UHPLC-MS/MS
por: Ding, Shujing, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_t2b6t7h4lka2o1uca60sv2eib9