Cargando…

The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes

Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Yang, Wu, Yiqi, Ji, Mengting, Rong, Xiaoyin, Zhang, Yanwei, Yang, Shuai, Lu, Chang, Cai, Chunbo, Gao, Pengfei, Guo, Xiaohong, Li, Bugao, Cao, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9552422/
https://www.ncbi.nlm.nih.gov/pubmed/36221052
http://dx.doi.org/10.1186/s12864-022-08923-9
_version_ 1784806247898284032
author Yang, Yang
Wu, Yiqi
Ji, Mengting
Rong, Xiaoyin
Zhang, Yanwei
Yang, Shuai
Lu, Chang
Cai, Chunbo
Gao, Pengfei
Guo, Xiaohong
Li, Bugao
Cao, Guoqing
author_facet Yang, Yang
Wu, Yiqi
Ji, Mengting
Rong, Xiaoyin
Zhang, Yanwei
Yang, Shuai
Lu, Chang
Cai, Chunbo
Gao, Pengfei
Guo, Xiaohong
Li, Bugao
Cao, Guoqing
author_sort Yang, Yang
collection PubMed
description Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more abundant in the adipose tissue of Mashen (fat-type) pigs than for Large White (lean-type) pigs, and knockdown of this lncRNA significantly inhibited the differentiation of porcine preadipocytes into adipocytes. Mechanistically, we used RNA pull-down and RIP assays to establish that lncMYOZ2 interacts with adenosylhomocysteinase (AHCY). Moreover, lncMYOZ2 knockdown increased promoter methylation of the target gene MYOZ2 and lowered its expression. Finally, we describe a positive regulatory role for MYOZ2 in adipogenesis. Collectively, these findings establish lncMYOZ2 as an important epigenetic regulator of adipogenesis via the aforementioned AHCY/MYOZ2 pathway, and provide insights into the role of lncRNAs in porcine adipose development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08923-9.
format Online
Article
Text
id pubmed-9552422
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-95524222022-10-12 The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes Yang, Yang Wu, Yiqi Ji, Mengting Rong, Xiaoyin Zhang, Yanwei Yang, Shuai Lu, Chang Cai, Chunbo Gao, Pengfei Guo, Xiaohong Li, Bugao Cao, Guoqing BMC Genomics Research Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more abundant in the adipose tissue of Mashen (fat-type) pigs than for Large White (lean-type) pigs, and knockdown of this lncRNA significantly inhibited the differentiation of porcine preadipocytes into adipocytes. Mechanistically, we used RNA pull-down and RIP assays to establish that lncMYOZ2 interacts with adenosylhomocysteinase (AHCY). Moreover, lncMYOZ2 knockdown increased promoter methylation of the target gene MYOZ2 and lowered its expression. Finally, we describe a positive regulatory role for MYOZ2 in adipogenesis. Collectively, these findings establish lncMYOZ2 as an important epigenetic regulator of adipogenesis via the aforementioned AHCY/MYOZ2 pathway, and provide insights into the role of lncRNAs in porcine adipose development. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08923-9. BioMed Central 2022-10-11 /pmc/articles/PMC9552422/ /pubmed/36221052 http://dx.doi.org/10.1186/s12864-022-08923-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yang, Yang
Wu, Yiqi
Ji, Mengting
Rong, Xiaoyin
Zhang, Yanwei
Yang, Shuai
Lu, Chang
Cai, Chunbo
Gao, Pengfei
Guo, Xiaohong
Li, Bugao
Cao, Guoqing
The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
title The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
title_full The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
title_fullStr The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
title_full_unstemmed The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
title_short The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
title_sort long non-coding rna lncmyoz2 mediates an ahcy/myoz2 axis to promote adipogenic differentiation in porcine preadipocytes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9552422/
https://www.ncbi.nlm.nih.gov/pubmed/36221052
http://dx.doi.org/10.1186/s12864-022-08923-9
work_keys_str_mv AT yangyang thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT wuyiqi thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT jimengting thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT rongxiaoyin thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT zhangyanwei thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT yangshuai thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT luchang thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT caichunbo thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT gaopengfei thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT guoxiaohong thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT libugao thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT caoguoqing thelongnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT yangyang longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT wuyiqi longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT jimengting longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT rongxiaoyin longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT zhangyanwei longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT yangshuai longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT luchang longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT caichunbo longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT gaopengfei longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT guoxiaohong longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT libugao longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes
AT caoguoqing longnoncodingrnalncmyoz2mediatesanahcymyoz2axistopromoteadipogenicdifferentiationinporcinepreadipocytes