Cargando…

Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)

BACKGROUND: To support bone regeneration, 3D-printed templates function as temporary guides. The preferred materials are synthetic polymers, due to their ease of processing and biological inertness. Poly(lactide-co-trimethylene carbonate) (PLATMC) has good biological compatibility and currently used...

Descripción completa

Detalles Bibliográficos
Autores principales: Hassan, Mohamad Nageeb, Yassin, Mohammed Ahmed, Eltawila, Ahmed Maher, Aladawi, Ahmed Emad, Mohamed-Ahmed, Samih, Suliman, Salwa, Kandil, Sherif, Mustafa, Kamal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9552430/
https://www.ncbi.nlm.nih.gov/pubmed/36217173
http://dx.doi.org/10.1186/s40824-022-00299-x
_version_ 1784806249949298688
author Hassan, Mohamad Nageeb
Yassin, Mohammed Ahmed
Eltawila, Ahmed Maher
Aladawi, Ahmed Emad
Mohamed-Ahmed, Samih
Suliman, Salwa
Kandil, Sherif
Mustafa, Kamal
author_facet Hassan, Mohamad Nageeb
Yassin, Mohammed Ahmed
Eltawila, Ahmed Maher
Aladawi, Ahmed Emad
Mohamed-Ahmed, Samih
Suliman, Salwa
Kandil, Sherif
Mustafa, Kamal
author_sort Hassan, Mohamad Nageeb
collection PubMed
description BACKGROUND: To support bone regeneration, 3D-printed templates function as temporary guides. The preferred materials are synthetic polymers, due to their ease of processing and biological inertness. Poly(lactide-co-trimethylene carbonate) (PLATMC) has good biological compatibility and currently used in soft tissue regeneration. The aim of this study was to evaluate the osteoconductivity of 3D-printed PLATMC templates for bone tissue engineering, in comparison with the widely used 3D-printed polycaprolactone (PCL) templates. METHODS: The printability and physical properties of 3D-printed templates were assessed, including wettability, tensile properties and the degradation profile. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were used to evaluate osteoconductivity and extracellular matrix secretion in vitro. In addition, 3D-printed templates were implanted in subcutaneous and calvarial bone defect models in rabbits. RESULTS: Compared to PCL, PLATMC exhibited greater wettability, strength, degradation, and promoted osteogenic differentiation of hBMSCs, with superior osteoconductivity. However, the higher ALP activity disclosed by PCL group at 7 and 21 days did not dictate better osteoconductivity. This was confirmed in vivo in the calvarial defect model, where PCL disclosed distant osteogenesis, while PLATMC disclosed greater areas of new bone and obvious contact osteogenesis on surface. CONCLUSIONS: This study shows for the first time the contact osteogenesis formed on a degradable synthetic co-polymer. 3D-printed PLATMC templates disclosed unique contact osteogenesis and significant higher amount of new bone regeneration, thus could be used to advantage in bone tissue engineering. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-022-00299-x.
format Online
Article
Text
id pubmed-9552430
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-95524302022-10-12 Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate) Hassan, Mohamad Nageeb Yassin, Mohammed Ahmed Eltawila, Ahmed Maher Aladawi, Ahmed Emad Mohamed-Ahmed, Samih Suliman, Salwa Kandil, Sherif Mustafa, Kamal Biomater Res Research Article BACKGROUND: To support bone regeneration, 3D-printed templates function as temporary guides. The preferred materials are synthetic polymers, due to their ease of processing and biological inertness. Poly(lactide-co-trimethylene carbonate) (PLATMC) has good biological compatibility and currently used in soft tissue regeneration. The aim of this study was to evaluate the osteoconductivity of 3D-printed PLATMC templates for bone tissue engineering, in comparison with the widely used 3D-printed polycaprolactone (PCL) templates. METHODS: The printability and physical properties of 3D-printed templates were assessed, including wettability, tensile properties and the degradation profile. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were used to evaluate osteoconductivity and extracellular matrix secretion in vitro. In addition, 3D-printed templates were implanted in subcutaneous and calvarial bone defect models in rabbits. RESULTS: Compared to PCL, PLATMC exhibited greater wettability, strength, degradation, and promoted osteogenic differentiation of hBMSCs, with superior osteoconductivity. However, the higher ALP activity disclosed by PCL group at 7 and 21 days did not dictate better osteoconductivity. This was confirmed in vivo in the calvarial defect model, where PCL disclosed distant osteogenesis, while PLATMC disclosed greater areas of new bone and obvious contact osteogenesis on surface. CONCLUSIONS: This study shows for the first time the contact osteogenesis formed on a degradable synthetic co-polymer. 3D-printed PLATMC templates disclosed unique contact osteogenesis and significant higher amount of new bone regeneration, thus could be used to advantage in bone tissue engineering. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40824-022-00299-x. BioMed Central 2022-10-10 /pmc/articles/PMC9552430/ /pubmed/36217173 http://dx.doi.org/10.1186/s40824-022-00299-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Hassan, Mohamad Nageeb
Yassin, Mohammed Ahmed
Eltawila, Ahmed Maher
Aladawi, Ahmed Emad
Mohamed-Ahmed, Samih
Suliman, Salwa
Kandil, Sherif
Mustafa, Kamal
Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)
title Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)
title_full Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)
title_fullStr Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)
title_full_unstemmed Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)
title_short Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate)
title_sort contact osteogenesis by biodegradable 3d-printed poly(lactide-co-trimethylene carbonate)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9552430/
https://www.ncbi.nlm.nih.gov/pubmed/36217173
http://dx.doi.org/10.1186/s40824-022-00299-x
work_keys_str_mv AT hassanmohamadnageeb contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT yassinmohammedahmed contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT eltawilaahmedmaher contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT aladawiahmedemad contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT mohamedahmedsamih contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT sulimansalwa contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT kandilsherif contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate
AT mustafakamal contactosteogenesisbybiodegradable3dprintedpolylactidecotrimethylenecarbonate