Cargando…

Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis

CONTEXT: Guanxin V (GX), a traditional Chinese medicine formula, is safe and effective in the treatment of coronary artery disease. However, its protective effect on myocardial ischaemia reperfusion injury (MIRI) is unclear. OBJECTIVE: To investigate the cardioprotective effect of GX on MIRI and exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Fuqiong, Zhang, Zhengguang, Wang, Meiyuan, Zhu, Weina, Ruan, Jie, Long, Hongyan, Zhang, Yajie, Gu, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553176/
https://www.ncbi.nlm.nih.gov/pubmed/36215067
http://dx.doi.org/10.1080/13880209.2022.2123934
_version_ 1784806408975286272
author Zhou, Fuqiong
Zhang, Zhengguang
Wang, Meiyuan
Zhu, Weina
Ruan, Jie
Long, Hongyan
Zhang, Yajie
Gu, Ning
author_facet Zhou, Fuqiong
Zhang, Zhengguang
Wang, Meiyuan
Zhu, Weina
Ruan, Jie
Long, Hongyan
Zhang, Yajie
Gu, Ning
author_sort Zhou, Fuqiong
collection PubMed
description CONTEXT: Guanxin V (GX), a traditional Chinese medicine formula, is safe and effective in the treatment of coronary artery disease. However, its protective effect on myocardial ischaemia reperfusion injury (MIRI) is unclear. OBJECTIVE: To investigate the cardioprotective effect of GX on MIRI and explore the potential mechanism. MATERIALS AND METHODS: Sprague-Dawley male rats were divided into Sham, MIRI and MIRI + GX groups. GX (6 g/kg) was administered to rats via intragastric administration for seven days before ischaemia reperfusion (IR) surgery. The infarct size, histopathology, serum enzyme activities, ultrastructure of the cardiac mitochondria were assessed. H9c2 cells were pre-treated with GX (0.5 mg/mL), and then exposed to hypoxia/reoxygenation (HR). The cell viability and LDH levels were measured. Network pharmacology was conducted to predict the potential mechanism. The related targets of GX were predicted using the TCMSP database, DrugBank database, etc. Finally, pharmacological experiments were used to validate the predicted results. RESULTS: In vivo, GX significantly reduced the myocardial infarct size from 56.33% to 17.18%, decreased the levels of AST (239.32 vs. 369.18 U/L), CK-MB (1324.61 vs. 2066.47 U/L) and LDH (1245.26 vs. 1969.62 U/L), and reduced mitochondrial damage. In vitro, GX significantly increased H9c2 cell viability (IC(50) = 3.913 mg/mL) and inhibited the release of LDH (207.35 vs. 314.33). In addition, GX could maintain iron homeostasis and reduce oxidative stress level by regulating iron metabolism-associated proteins. CONCLUSIONS: GX can attenuate MIRI via regulating iron homeostasis, indicating that GX may act as a potential candidate for the treatment of MIRI.
format Online
Article
Text
id pubmed-9553176
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-95531762022-10-12 Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis Zhou, Fuqiong Zhang, Zhengguang Wang, Meiyuan Zhu, Weina Ruan, Jie Long, Hongyan Zhang, Yajie Gu, Ning Pharm Biol Research Article CONTEXT: Guanxin V (GX), a traditional Chinese medicine formula, is safe and effective in the treatment of coronary artery disease. However, its protective effect on myocardial ischaemia reperfusion injury (MIRI) is unclear. OBJECTIVE: To investigate the cardioprotective effect of GX on MIRI and explore the potential mechanism. MATERIALS AND METHODS: Sprague-Dawley male rats were divided into Sham, MIRI and MIRI + GX groups. GX (6 g/kg) was administered to rats via intragastric administration for seven days before ischaemia reperfusion (IR) surgery. The infarct size, histopathology, serum enzyme activities, ultrastructure of the cardiac mitochondria were assessed. H9c2 cells were pre-treated with GX (0.5 mg/mL), and then exposed to hypoxia/reoxygenation (HR). The cell viability and LDH levels were measured. Network pharmacology was conducted to predict the potential mechanism. The related targets of GX were predicted using the TCMSP database, DrugBank database, etc. Finally, pharmacological experiments were used to validate the predicted results. RESULTS: In vivo, GX significantly reduced the myocardial infarct size from 56.33% to 17.18%, decreased the levels of AST (239.32 vs. 369.18 U/L), CK-MB (1324.61 vs. 2066.47 U/L) and LDH (1245.26 vs. 1969.62 U/L), and reduced mitochondrial damage. In vitro, GX significantly increased H9c2 cell viability (IC(50) = 3.913 mg/mL) and inhibited the release of LDH (207.35 vs. 314.33). In addition, GX could maintain iron homeostasis and reduce oxidative stress level by regulating iron metabolism-associated proteins. CONCLUSIONS: GX can attenuate MIRI via regulating iron homeostasis, indicating that GX may act as a potential candidate for the treatment of MIRI. Taylor & Francis 2022-10-10 /pmc/articles/PMC9553176/ /pubmed/36215067 http://dx.doi.org/10.1080/13880209.2022.2123934 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Fuqiong
Zhang, Zhengguang
Wang, Meiyuan
Zhu, Weina
Ruan, Jie
Long, Hongyan
Zhang, Yajie
Gu, Ning
Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
title Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
title_full Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
title_fullStr Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
title_full_unstemmed Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
title_short Guanxin V attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
title_sort guanxin v attenuates myocardial ischaemia reperfusion injury through regulating iron homeostasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553176/
https://www.ncbi.nlm.nih.gov/pubmed/36215067
http://dx.doi.org/10.1080/13880209.2022.2123934
work_keys_str_mv AT zhoufuqiong guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT zhangzhengguang guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT wangmeiyuan guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT zhuweina guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT ruanjie guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT longhongyan guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT zhangyajie guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis
AT guning guanxinvattenuatesmyocardialischaemiareperfusioninjurythroughregulatingironhomeostasis