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Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells

PURPOSE: The eyes are one of the most frequently involved organs in sarcoidosis in Asia, including Japan. Sarcoid uveitis is the major complaint of ocular sarcoidosis. The detection of epithelioid granuloma (EG) requires histological biopsy of the uvea for the precise diagnosis of sarcoid uveitis, b...

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Autores principales: Ohe, Rintaro, Kaneko, Yutaka, Namba, Hiroyuki, Nishi, Katsuhiro, Goto, Jun-Ichi, Futakuchi, Mitsuru, Nishitsuka, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553316/
https://www.ncbi.nlm.nih.gov/pubmed/36237490
http://dx.doi.org/10.2147/OPTH.S376141
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author Ohe, Rintaro
Kaneko, Yutaka
Namba, Hiroyuki
Nishi, Katsuhiro
Goto, Jun-Ichi
Futakuchi, Mitsuru
Nishitsuka, Koichi
author_facet Ohe, Rintaro
Kaneko, Yutaka
Namba, Hiroyuki
Nishi, Katsuhiro
Goto, Jun-Ichi
Futakuchi, Mitsuru
Nishitsuka, Koichi
author_sort Ohe, Rintaro
collection PubMed
description PURPOSE: The eyes are one of the most frequently involved organs in sarcoidosis in Asia, including Japan. Sarcoid uveitis is the major complaint of ocular sarcoidosis. The detection of epithelioid granuloma (EG) requires histological biopsy of the uvea for the precise diagnosis of sarcoid uveitis, because it is challenging to diagnose sarcoid uveitis without a history of systemic sarcoidosis. To diagnose sarcoid uveitis, we have established novel methods. PATIENTS AND METHODS: In this study, we included 30 eyes of 21 patients with granulomatous uveitis diagnosed via slit-lamp examinations, gonioscopy, fundus photography, and fluorescein angiography. Vitrectomy was performed to remove the vitreous opacity with vision loss. To examine vitreous cell components, we used liquid-based cytology (LBC). To detect EG in an intraocular irrigating solution, we collected vitreous cell components, and then the cell pellets were embedded in the cell block procedure. RESULTS: Here, we demonstrated the usefulness of the histological detection of EG and epithelioid cells (ECs) in LBC from vitreous body specimens and in the cell block procedure from vitreous cell components in an intraocular irrigating solution. Our results showed that the detection rates of EG were 6.3% (1/16) in LBC and 9.1% (1/11) in the cell block procedure in the sarcoid uveitis-suspected group and 7.7% (1/13) in LBC and 28.6% (2/7) in the cell block procedure in the sarcoidosis group. We would discuss the specificity of the EG/EC detection of ocular sarcoidosis. CONCLUSION: Our methods are helpful in the precise diagnosis of ocular sarcoidosis and the control of the development of systemic sarcoidosis.
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spelling pubmed-95533162022-10-12 Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells Ohe, Rintaro Kaneko, Yutaka Namba, Hiroyuki Nishi, Katsuhiro Goto, Jun-Ichi Futakuchi, Mitsuru Nishitsuka, Koichi Clin Ophthalmol Original Research PURPOSE: The eyes are one of the most frequently involved organs in sarcoidosis in Asia, including Japan. Sarcoid uveitis is the major complaint of ocular sarcoidosis. The detection of epithelioid granuloma (EG) requires histological biopsy of the uvea for the precise diagnosis of sarcoid uveitis, because it is challenging to diagnose sarcoid uveitis without a history of systemic sarcoidosis. To diagnose sarcoid uveitis, we have established novel methods. PATIENTS AND METHODS: In this study, we included 30 eyes of 21 patients with granulomatous uveitis diagnosed via slit-lamp examinations, gonioscopy, fundus photography, and fluorescein angiography. Vitrectomy was performed to remove the vitreous opacity with vision loss. To examine vitreous cell components, we used liquid-based cytology (LBC). To detect EG in an intraocular irrigating solution, we collected vitreous cell components, and then the cell pellets were embedded in the cell block procedure. RESULTS: Here, we demonstrated the usefulness of the histological detection of EG and epithelioid cells (ECs) in LBC from vitreous body specimens and in the cell block procedure from vitreous cell components in an intraocular irrigating solution. Our results showed that the detection rates of EG were 6.3% (1/16) in LBC and 9.1% (1/11) in the cell block procedure in the sarcoid uveitis-suspected group and 7.7% (1/13) in LBC and 28.6% (2/7) in the cell block procedure in the sarcoidosis group. We would discuss the specificity of the EG/EC detection of ocular sarcoidosis. CONCLUSION: Our methods are helpful in the precise diagnosis of ocular sarcoidosis and the control of the development of systemic sarcoidosis. Dove 2022-10-10 /pmc/articles/PMC9553316/ /pubmed/36237490 http://dx.doi.org/10.2147/OPTH.S376141 Text en © 2022 Ohe et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Ohe, Rintaro
Kaneko, Yutaka
Namba, Hiroyuki
Nishi, Katsuhiro
Goto, Jun-Ichi
Futakuchi, Mitsuru
Nishitsuka, Koichi
Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells
title Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells
title_full Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells
title_fullStr Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells
title_full_unstemmed Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells
title_short Utility of Liquid-Based Cytology and Cell Block Procedure Obtained by Vitrectomy to Diagnose Ocular Sarcoidosis–The Significance of Epithelioid Granuloma and Epithelioid Cells
title_sort utility of liquid-based cytology and cell block procedure obtained by vitrectomy to diagnose ocular sarcoidosis–the significance of epithelioid granuloma and epithelioid cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553316/
https://www.ncbi.nlm.nih.gov/pubmed/36237490
http://dx.doi.org/10.2147/OPTH.S376141
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