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In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

METHODS: The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in...

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Autores principales: Akbari, Fatemeh, Azadbakht, Mohammad, Bagheri, Ali, Vahedi, Lale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553362/
https://www.ncbi.nlm.nih.gov/pubmed/36238201
http://dx.doi.org/10.1155/2022/2156629
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author Akbari, Fatemeh
Azadbakht, Mohammad
Bagheri, Ali
Vahedi, Lale
author_facet Akbari, Fatemeh
Azadbakht, Mohammad
Bagheri, Ali
Vahedi, Lale
author_sort Akbari, Fatemeh
collection PubMed
description METHODS: The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in vitro (NHDF cell line, by 500 μg/ml concentration of the extract, 24 h) and in vivo (Wistar rat, topical daily treated with 1.5% of the extract ointment, 21 days) conditions in comparison to control groups was conducted. Rats' control groups included silver sulfadiazine, Vaseline, and the nontreated groups. RESULTS: Eleven flavonoids were detected using LC/MS. The in silico study showed that formononetin, kaempferol-based structures, quercetin-3-O-neohesperidoside, and calycosin-7-O-beta-D-glucoside had a high affinity (<−6.3) to ERs α and β. Wound closing measurement showed significant improvement in the group treated with the extract in both in vitro and in vivo assays compared to the control groups. Histopathological results confirmed these findings; inflammation factors decreased, and fibroblast proliferation, fibrosis, and epithelization increased, especially in the extract group. CONCLUSION: This study shows that Astragalus microcephalus has wound healing activity in vitro and in vivo with low toxicity due to the presence of flavonoids, especially isoflavonoids, which show a high affinity to bind to ERs α and β in the skin tissue.
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spelling pubmed-95533622022-10-12 In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. Akbari, Fatemeh Azadbakht, Mohammad Bagheri, Ali Vahedi, Lale Adv Pharmacol Pharm Sci Research Article METHODS: The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in vitro (NHDF cell line, by 500 μg/ml concentration of the extract, 24 h) and in vivo (Wistar rat, topical daily treated with 1.5% of the extract ointment, 21 days) conditions in comparison to control groups was conducted. Rats' control groups included silver sulfadiazine, Vaseline, and the nontreated groups. RESULTS: Eleven flavonoids were detected using LC/MS. The in silico study showed that formononetin, kaempferol-based structures, quercetin-3-O-neohesperidoside, and calycosin-7-O-beta-D-glucoside had a high affinity (<−6.3) to ERs α and β. Wound closing measurement showed significant improvement in the group treated with the extract in both in vitro and in vivo assays compared to the control groups. Histopathological results confirmed these findings; inflammation factors decreased, and fibroblast proliferation, fibrosis, and epithelization increased, especially in the extract group. CONCLUSION: This study shows that Astragalus microcephalus has wound healing activity in vitro and in vivo with low toxicity due to the presence of flavonoids, especially isoflavonoids, which show a high affinity to bind to ERs α and β in the skin tissue. Hindawi 2022-10-04 /pmc/articles/PMC9553362/ /pubmed/36238201 http://dx.doi.org/10.1155/2022/2156629 Text en Copyright © 2022 Fatemeh Akbari et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Akbari, Fatemeh
Azadbakht, Mohammad
Bagheri, Ali
Vahedi, Lale
In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
title In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
title_full In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
title_fullStr In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
title_full_unstemmed In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
title_short In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.
title_sort in silico, in vitro, and in vivo wound healing activity of astragalus microcephalus willd.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553362/
https://www.ncbi.nlm.nih.gov/pubmed/36238201
http://dx.doi.org/10.1155/2022/2156629
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