Cargando…

Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway

Upregulation of keratin 80 (KRT80) expression levels and carcinogenic function has been found in several types of tumors. However, its contribution and mechanism in NSCLC remain to be outlined. In this study, bioinformatic investigation from the TCGA dataset revealed that KRT80 was confirmed to be e...

Descripción completa

Detalles Bibliográficos
Autores principales: Tong, Yueyang, Chen, Xueyuan, Feng, Zhemin, Xu, Changqing, Li, Yaqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553464/
https://www.ncbi.nlm.nih.gov/pubmed/36248424
http://dx.doi.org/10.1155/2022/2630351
_version_ 1784806477284769792
author Tong, Yueyang
Chen, Xueyuan
Feng, Zhemin
Xu, Changqing
Li, Yaqian
author_facet Tong, Yueyang
Chen, Xueyuan
Feng, Zhemin
Xu, Changqing
Li, Yaqian
author_sort Tong, Yueyang
collection PubMed
description Upregulation of keratin 80 (KRT80) expression levels and carcinogenic function has been found in several types of tumors. However, its contribution and mechanism in NSCLC remain to be outlined. In this study, bioinformatic investigation from the TCGA dataset revealed that KRT80 was confirmed to be elevated in human NSCLC tissues. The results of qRT-PCR and Western blot assays disclosed that KRT80 was uplifted in NSCLC cells. Data from CCK-8 and colony formation assays exhibited that depletion of KRT80 restrained NSCLC cell proliferation. Findings from Transwell and Western blot assays illustrated that downregulation of KRT80 inhibited NSCLC cell migration, invasion, and EMT. Further mechanism exploration implied that KRT80 may be included within the regulation of EMT of NSCLC cells by affecting the TGF-β/SMAD pathway. Moreover, depletion of KRT80 attenuated xenograft tumor growth and the expressions of KRT80, Ki-67, and TGFBR1. In conclusion, depletion of KRT80 repressed NSCLC cell proliferation, invasion, and EMT, possibly mediated by the TGF-β/SMAD signaling pathway, indicating that KRT80 may be a potentially useful target for NSCLC.
format Online
Article
Text
id pubmed-9553464
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-95534642022-10-13 Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway Tong, Yueyang Chen, Xueyuan Feng, Zhemin Xu, Changqing Li, Yaqian Evid Based Complement Alternat Med Research Article Upregulation of keratin 80 (KRT80) expression levels and carcinogenic function has been found in several types of tumors. However, its contribution and mechanism in NSCLC remain to be outlined. In this study, bioinformatic investigation from the TCGA dataset revealed that KRT80 was confirmed to be elevated in human NSCLC tissues. The results of qRT-PCR and Western blot assays disclosed that KRT80 was uplifted in NSCLC cells. Data from CCK-8 and colony formation assays exhibited that depletion of KRT80 restrained NSCLC cell proliferation. Findings from Transwell and Western blot assays illustrated that downregulation of KRT80 inhibited NSCLC cell migration, invasion, and EMT. Further mechanism exploration implied that KRT80 may be included within the regulation of EMT of NSCLC cells by affecting the TGF-β/SMAD pathway. Moreover, depletion of KRT80 attenuated xenograft tumor growth and the expressions of KRT80, Ki-67, and TGFBR1. In conclusion, depletion of KRT80 repressed NSCLC cell proliferation, invasion, and EMT, possibly mediated by the TGF-β/SMAD signaling pathway, indicating that KRT80 may be a potentially useful target for NSCLC. Hindawi 2022-09-19 /pmc/articles/PMC9553464/ /pubmed/36248424 http://dx.doi.org/10.1155/2022/2630351 Text en Copyright © 2022 Yueyang Tong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tong, Yueyang
Chen, Xueyuan
Feng, Zhemin
Xu, Changqing
Li, Yaqian
Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway
title Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway
title_full Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway
title_fullStr Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway
title_full_unstemmed Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway
title_short Keratin 80 Promotes Migration and Invasion of Non-Small Cell Lung Cancer Cells by Regulating the TGF-β/SMAD Pathway
title_sort keratin 80 promotes migration and invasion of non-small cell lung cancer cells by regulating the tgf-β/smad pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553464/
https://www.ncbi.nlm.nih.gov/pubmed/36248424
http://dx.doi.org/10.1155/2022/2630351
work_keys_str_mv AT tongyueyang keratin80promotesmigrationandinvasionofnonsmallcelllungcancercellsbyregulatingthetgfbsmadpathway
AT chenxueyuan keratin80promotesmigrationandinvasionofnonsmallcelllungcancercellsbyregulatingthetgfbsmadpathway
AT fengzhemin keratin80promotesmigrationandinvasionofnonsmallcelllungcancercellsbyregulatingthetgfbsmadpathway
AT xuchangqing keratin80promotesmigrationandinvasionofnonsmallcelllungcancercellsbyregulatingthetgfbsmadpathway
AT liyaqian keratin80promotesmigrationandinvasionofnonsmallcelllungcancercellsbyregulatingthetgfbsmadpathway