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Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect

Cartilage is an alymphatic, avascular and non-innervated tissue. Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair. We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosa...

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Autores principales: Wei, Li, Qin, Shuai, Ye, Yulin, Hu, Jiawei, Luo, Danyang, Li, Yusi, Gao, Yiming, Jiang, Liting, Zhou, Qi, Xie, Xianfei, Li, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553786/
https://www.ncbi.nlm.nih.gov/pubmed/36219265
http://dx.doi.org/10.1007/s10856-022-06695-y
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author Wei, Li
Qin, Shuai
Ye, Yulin
Hu, Jiawei
Luo, Danyang
Li, Yusi
Gao, Yiming
Jiang, Liting
Zhou, Qi
Xie, Xianfei
Li, Ning
author_facet Wei, Li
Qin, Shuai
Ye, Yulin
Hu, Jiawei
Luo, Danyang
Li, Yusi
Gao, Yiming
Jiang, Liting
Zhou, Qi
Xie, Xianfei
Li, Ning
author_sort Wei, Li
collection PubMed
description Cartilage is an alymphatic, avascular and non-innervated tissue. Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair. We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure. The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility. Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture. The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes. Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role. Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair. These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application.
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spelling pubmed-95537862022-10-13 Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect Wei, Li Qin, Shuai Ye, Yulin Hu, Jiawei Luo, Danyang Li, Yusi Gao, Yiming Jiang, Liting Zhou, Qi Xie, Xianfei Li, Ning J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates Cartilage is an alymphatic, avascular and non-innervated tissue. Lack of potential regenerative capacity to reconstruct chondral defect has accelerated investigation and development of new strategy for cartilage repair. We prepared a manganese ion-incorporated natupolymer-based scaffold with chitosan-gelatin by freeze-drying procedure. The scaffold was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, energy dispersive spectroscopy, compressive testing, and analysis of porosity and flexibility. Live/dead assay confirmed the good cytocompatibility of prepared scaffold on rat articular chondrocytes after 10 days and 4 weeks of culture. The manganese-loaded composite scaffold upregulated the expression of chondrogenic-related markers (Sox9, integrin, and Col II) in chondrocytes. Western blot analysis of proteins extracted from chondrocytes grown on scaffolds indicated the signaling pathways of p-Akt and p-ERK1/2 played a key role. Histological analysis following implantation of current composite scaffold loaded with chondrocytes into a rat articular cartilage defect model showed that the scaffolds promoted the formation of collagen II and cartilage repair. These findings suggested the potential of manganese-loaded scaffold to promote new cartilage formation and a promising strategy for articular cartilage engineering application. Springer US 2022-10-11 2022 /pmc/articles/PMC9553786/ /pubmed/36219265 http://dx.doi.org/10.1007/s10856-022-06695-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Tissue Engineering Constructs and Cell Substrates
Wei, Li
Qin, Shuai
Ye, Yulin
Hu, Jiawei
Luo, Danyang
Li, Yusi
Gao, Yiming
Jiang, Liting
Zhou, Qi
Xie, Xianfei
Li, Ning
Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
title Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
title_full Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
title_fullStr Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
title_full_unstemmed Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
title_short Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
title_sort chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defect
topic Tissue Engineering Constructs and Cell Substrates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553786/
https://www.ncbi.nlm.nih.gov/pubmed/36219265
http://dx.doi.org/10.1007/s10856-022-06695-y
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