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Metabolite profiling of Arabidopsis mutants of lower glycolysis
We have previously shown that in Arabidopsis the three enzymes of lower glycolysis namely phosphoglycerate mutase (PGAM), enolase and pyruvate kinase form a complex which plays an important role in tethering the mitochondria to the chloroplast. Given that the metabolism of these mutants, the complem...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553893/ https://www.ncbi.nlm.nih.gov/pubmed/36220829 http://dx.doi.org/10.1038/s41597-022-01673-z |
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author | Zhang, Youjun Fernie, Alisdair R. |
author_facet | Zhang, Youjun Fernie, Alisdair R. |
author_sort | Zhang, Youjun |
collection | PubMed |
description | We have previously shown that in Arabidopsis the three enzymes of lower glycolysis namely phosphoglycerate mutase (PGAM), enolase and pyruvate kinase form a complex which plays an important role in tethering the mitochondria to the chloroplast. Given that the metabolism of these mutants, the complemented of pgam mutant and overexpression lines of PGAM were unclear, here, we present gas chromatography mass spectrometry-based metabolomics data of them alongside their plant growth phenotypes. Compared with wild type, both sugar and amino acid concentration are significantly altered in phosphoglycerate mutase, enolase and pyruvate kinase. Conversely, overexpression of PGAM could decrease the content of 3PGA, sugar and several amino acids and increase the content of alanine and pyruvate. In addition, the pgam mutant could not be fully complemented by either a nuclear target pgam, a side-directed-mutate of pgam or a the E.coli PGAM in term of plant phenotype or metabolite profiles, suggesting the low glycolysis complete formation is required to support normal metabolism and growth. |
format | Online Article Text |
id | pubmed-9553893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95538932022-10-13 Metabolite profiling of Arabidopsis mutants of lower glycolysis Zhang, Youjun Fernie, Alisdair R. Sci Data Data Descriptor We have previously shown that in Arabidopsis the three enzymes of lower glycolysis namely phosphoglycerate mutase (PGAM), enolase and pyruvate kinase form a complex which plays an important role in tethering the mitochondria to the chloroplast. Given that the metabolism of these mutants, the complemented of pgam mutant and overexpression lines of PGAM were unclear, here, we present gas chromatography mass spectrometry-based metabolomics data of them alongside their plant growth phenotypes. Compared with wild type, both sugar and amino acid concentration are significantly altered in phosphoglycerate mutase, enolase and pyruvate kinase. Conversely, overexpression of PGAM could decrease the content of 3PGA, sugar and several amino acids and increase the content of alanine and pyruvate. In addition, the pgam mutant could not be fully complemented by either a nuclear target pgam, a side-directed-mutate of pgam or a the E.coli PGAM in term of plant phenotype or metabolite profiles, suggesting the low glycolysis complete formation is required to support normal metabolism and growth. Nature Publishing Group UK 2022-10-11 /pmc/articles/PMC9553893/ /pubmed/36220829 http://dx.doi.org/10.1038/s41597-022-01673-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Data Descriptor Zhang, Youjun Fernie, Alisdair R. Metabolite profiling of Arabidopsis mutants of lower glycolysis |
title | Metabolite profiling of Arabidopsis mutants of lower glycolysis |
title_full | Metabolite profiling of Arabidopsis mutants of lower glycolysis |
title_fullStr | Metabolite profiling of Arabidopsis mutants of lower glycolysis |
title_full_unstemmed | Metabolite profiling of Arabidopsis mutants of lower glycolysis |
title_short | Metabolite profiling of Arabidopsis mutants of lower glycolysis |
title_sort | metabolite profiling of arabidopsis mutants of lower glycolysis |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9553893/ https://www.ncbi.nlm.nih.gov/pubmed/36220829 http://dx.doi.org/10.1038/s41597-022-01673-z |
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