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Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress

Pinus massoniana Lamb. is the timber species with the widest distribution and the largest afforestation area in China, providing a large amount of timber, turpentine and ecological products. but low temperature limits its growth and geographical distribution. Physiological and molecular studies can...

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Autores principales: Lu, Jingyu, Chen, Hu, Yang, Zhangqi, Sun, Shuang, Luo, Qunfeng, Xie, Junkang, Tan, Jianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554314/
https://www.ncbi.nlm.nih.gov/pubmed/36247576
http://dx.doi.org/10.3389/fpls.2022.954324
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author Lu, Jingyu
Chen, Hu
Yang, Zhangqi
Sun, Shuang
Luo, Qunfeng
Xie, Junkang
Tan, Jianhui
author_facet Lu, Jingyu
Chen, Hu
Yang, Zhangqi
Sun, Shuang
Luo, Qunfeng
Xie, Junkang
Tan, Jianhui
author_sort Lu, Jingyu
collection PubMed
description Pinus massoniana Lamb. is the timber species with the widest distribution and the largest afforestation area in China, providing a large amount of timber, turpentine and ecological products. but low temperature limits its growth and geographical distribution. Physiological and molecular studies can well explain the mechanism of P. massoniana response to low temperature. In this study, physiological and biochemical indexes, cell morphology, lignin content, gene regulatory networks, and gene expression patterns of different P. massoniana varieties (cold-tolerant and cold-sensitive) were studied from physiological, biochemical, and molecular perspectives. The results indicated that under low-temperature stress, the cold-tolerant cultivar maintained high contents of osmoregulatory substances, and the root morphology and structure remained intact. In the initial stage of low-temperature stress, the number of differentially expressed genes was 7148, and with the extension of stress time, the number of differentially expressed genes decreased to 1991. P. massoniana might direct its responses to low temperature by regulating phenylpropane metabolism, starch and sucrose metabolism, hormone signaling pathways, and transcription factors. BAM, 4CL, CCoAOMT, PRX5, WRKYs, and hormone synthesis related genes play important roles. P. massoniana cultivars may vary in response mechanisms. In this study, physiological and analytical techniques were used to study the root tip response mechanism of Masson’s pine to low temperature stress. The results of this study lay a foundation for in-depth research on the molecular functions of P. massoniana under low-temperature stress conditions.
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spelling pubmed-95543142022-10-13 Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress Lu, Jingyu Chen, Hu Yang, Zhangqi Sun, Shuang Luo, Qunfeng Xie, Junkang Tan, Jianhui Front Plant Sci Plant Science Pinus massoniana Lamb. is the timber species with the widest distribution and the largest afforestation area in China, providing a large amount of timber, turpentine and ecological products. but low temperature limits its growth and geographical distribution. Physiological and molecular studies can well explain the mechanism of P. massoniana response to low temperature. In this study, physiological and biochemical indexes, cell morphology, lignin content, gene regulatory networks, and gene expression patterns of different P. massoniana varieties (cold-tolerant and cold-sensitive) were studied from physiological, biochemical, and molecular perspectives. The results indicated that under low-temperature stress, the cold-tolerant cultivar maintained high contents of osmoregulatory substances, and the root morphology and structure remained intact. In the initial stage of low-temperature stress, the number of differentially expressed genes was 7148, and with the extension of stress time, the number of differentially expressed genes decreased to 1991. P. massoniana might direct its responses to low temperature by regulating phenylpropane metabolism, starch and sucrose metabolism, hormone signaling pathways, and transcription factors. BAM, 4CL, CCoAOMT, PRX5, WRKYs, and hormone synthesis related genes play important roles. P. massoniana cultivars may vary in response mechanisms. In this study, physiological and analytical techniques were used to study the root tip response mechanism of Masson’s pine to low temperature stress. The results of this study lay a foundation for in-depth research on the molecular functions of P. massoniana under low-temperature stress conditions. Frontiers Media S.A. 2022-09-28 /pmc/articles/PMC9554314/ /pubmed/36247576 http://dx.doi.org/10.3389/fpls.2022.954324 Text en Copyright © 2022 Lu, Chen, Yang, Sun, Luo, Xie and Tan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lu, Jingyu
Chen, Hu
Yang, Zhangqi
Sun, Shuang
Luo, Qunfeng
Xie, Junkang
Tan, Jianhui
Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress
title Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress
title_full Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress
title_fullStr Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress
title_full_unstemmed Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress
title_short Physiological and molecular mechanisms of the response of roots of Pinus massoniana Lamb. to low-temperature stress
title_sort physiological and molecular mechanisms of the response of roots of pinus massoniana lamb. to low-temperature stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554314/
https://www.ncbi.nlm.nih.gov/pubmed/36247576
http://dx.doi.org/10.3389/fpls.2022.954324
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