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Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)

In order to improve the glucose utilization capacity of largemouth bass (Micropterus salmoides), responses to glucose overload between two strains (Y: breeding strain; W: wild strain) were compared at 0, 6, 12, and 24 h after glucose injection (1.67 g/kg). The data revealed that plasma glucose in th...

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Autores principales: Lei, Caixia, Xie, Yujing, Song, Hongmei, Jiang, Peng, Du, Jinxing, Li, Shengjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554351/
https://www.ncbi.nlm.nih.gov/pubmed/36246125
http://dx.doi.org/10.3389/fphys.2022.1010633
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author Lei, Caixia
Xie, Yujing
Song, Hongmei
Jiang, Peng
Du, Jinxing
Li, Shengjie
author_facet Lei, Caixia
Xie, Yujing
Song, Hongmei
Jiang, Peng
Du, Jinxing
Li, Shengjie
author_sort Lei, Caixia
collection PubMed
description In order to improve the glucose utilization capacity of largemouth bass (Micropterus salmoides), responses to glucose overload between two strains (Y: breeding strain; W: wild strain) were compared at 0, 6, 12, and 24 h after glucose injection (1.67 g/kg). The data revealed that plasma glucose in the Y strain (<12 h) recovered faster than in the W strain (12 h), with the Y strain secreted more insulin within 6 h post-injection. Triglyceride (TG) and low-density lipoprotein-cholesterol (VLDL-CH) content in the Y strain increased, peaking at 12 h, then decreased, whereas the W strain’s TG content was not affected and VLDL-CH content decreased. The hepatic and muscular fatty acid synthetase, liver x receptor-1, and sterol regulatory element-binding protein expressions were consistent with the TG content change. Both strains’ liver and muscle glycogen contents exhibited similar trends to that of the glycogen synthase gene—increasing, then declining, and peaking at 6 and 12 h. The expression levels of hepatic and muscular phosphofructokinase and pyruvate kinase in the Y strain increased, peaking at 12 h. In the W strain, they were suppressed and reached the minimum at 24 h. The mRNA levels of hepatic and muscular phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were enhanced and peaked at 24 h in both strains, hepatic isocitrate dehydrogenase-1, and α-ketoglutarate dehydrogenase complex expression increased after declining, peaking at 12 and 24 h. Two genes in the W strain’s muscles showed a similar trend. Both strains’ transcriptome results identified seven common functional genes for resistance to hyperglycemia that were involved in the circadian rhythm pathway, which is a suggested key pathway for coping with hyperglycemia. Furthermore, 48 differential genes were identified between the two strains, and these genes were enriched in the TGF-beta and cell cycle signaling pathways, indicating that these pathways may be key factors affecting the differential responses to glucose overload. We conducted a comprehensive comparison of glucose overload molecular responses between two strains of M. salmoides, and the results can provide a promising strategy to improve the glucose utilization capacity of M. salmoides based on advantageous pre-existing traits.
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spelling pubmed-95543512022-10-13 Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides) Lei, Caixia Xie, Yujing Song, Hongmei Jiang, Peng Du, Jinxing Li, Shengjie Front Physiol Physiology In order to improve the glucose utilization capacity of largemouth bass (Micropterus salmoides), responses to glucose overload between two strains (Y: breeding strain; W: wild strain) were compared at 0, 6, 12, and 24 h after glucose injection (1.67 g/kg). The data revealed that plasma glucose in the Y strain (<12 h) recovered faster than in the W strain (12 h), with the Y strain secreted more insulin within 6 h post-injection. Triglyceride (TG) and low-density lipoprotein-cholesterol (VLDL-CH) content in the Y strain increased, peaking at 12 h, then decreased, whereas the W strain’s TG content was not affected and VLDL-CH content decreased. The hepatic and muscular fatty acid synthetase, liver x receptor-1, and sterol regulatory element-binding protein expressions were consistent with the TG content change. Both strains’ liver and muscle glycogen contents exhibited similar trends to that of the glycogen synthase gene—increasing, then declining, and peaking at 6 and 12 h. The expression levels of hepatic and muscular phosphofructokinase and pyruvate kinase in the Y strain increased, peaking at 12 h. In the W strain, they were suppressed and reached the minimum at 24 h. The mRNA levels of hepatic and muscular phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were enhanced and peaked at 24 h in both strains, hepatic isocitrate dehydrogenase-1, and α-ketoglutarate dehydrogenase complex expression increased after declining, peaking at 12 and 24 h. Two genes in the W strain’s muscles showed a similar trend. Both strains’ transcriptome results identified seven common functional genes for resistance to hyperglycemia that were involved in the circadian rhythm pathway, which is a suggested key pathway for coping with hyperglycemia. Furthermore, 48 differential genes were identified between the two strains, and these genes were enriched in the TGF-beta and cell cycle signaling pathways, indicating that these pathways may be key factors affecting the differential responses to glucose overload. We conducted a comprehensive comparison of glucose overload molecular responses between two strains of M. salmoides, and the results can provide a promising strategy to improve the glucose utilization capacity of M. salmoides based on advantageous pre-existing traits. Frontiers Media S.A. 2022-09-28 /pmc/articles/PMC9554351/ /pubmed/36246125 http://dx.doi.org/10.3389/fphys.2022.1010633 Text en Copyright © 2022 Lei, Xie, Song, Jiang, Du and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Lei, Caixia
Xie, Yujing
Song, Hongmei
Jiang, Peng
Du, Jinxing
Li, Shengjie
Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)
title Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)
title_full Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)
title_fullStr Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)
title_full_unstemmed Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)
title_short Different responses to glucose overload between two strains of largemouth bass (Micropterus salmoides)
title_sort different responses to glucose overload between two strains of largemouth bass (micropterus salmoides)
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554351/
https://www.ncbi.nlm.nih.gov/pubmed/36246125
http://dx.doi.org/10.3389/fphys.2022.1010633
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