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Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy

African swine fever (ASF) is a highly contagious hemorrhagic and transboundary animal disease, and it threatens global food security. A full necropsy to harvest the sample matrices for diagnosis in the farm may lead to contamination of the premises and directly threaten to the herds. In the present...

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Autores principales: Li, Xiaowen, Li, Yang, Fan, Mingyu, Fan, Shiran, Gao, Wenchao, Ren, Jing, Liu, Qingyuan, Li, Jingtao, Wu, Weisheng, Li, Junxian, Yu, Qiannan, Wang, Xinglong, Yan, Zhichun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554536/
https://www.ncbi.nlm.nih.gov/pubmed/36246330
http://dx.doi.org/10.3389/fvets.2022.1000969
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author Li, Xiaowen
Li, Yang
Fan, Mingyu
Fan, Shiran
Gao, Wenchao
Ren, Jing
Liu, Qingyuan
Li, Jingtao
Wu, Weisheng
Li, Junxian
Yu, Qiannan
Wang, Xinglong
Yan, Zhichun
author_facet Li, Xiaowen
Li, Yang
Fan, Mingyu
Fan, Shiran
Gao, Wenchao
Ren, Jing
Liu, Qingyuan
Li, Jingtao
Wu, Weisheng
Li, Junxian
Yu, Qiannan
Wang, Xinglong
Yan, Zhichun
author_sort Li, Xiaowen
collection PubMed
description African swine fever (ASF) is a highly contagious hemorrhagic and transboundary animal disease, and it threatens global food security. A full necropsy to harvest the sample matrices for diagnosis in the farm may lead to contamination of the premises and directly threaten to the herds. In the present study, we compared the ASFV loads of the common samples that can be collected without necropsy. The unmatched nasal, throat, rectal samples were randomly taken using cotton swabs, and inguinal lymph node samples were collected by the minimally invasive samplers from the dead pigs of an ASF field outbreak farm. The ASFV loads of the samples were detected by qPCR and the results suggested that the overall ASFV nucleic acids levels of inguinal lymph node samples were higher than the swabs. What's more, sets of matched nasal swabs, rectal swabs, throat swabs, inguinal lymph nodes, serums, spleens and lungs samples were collected from 15 dead ASFV naturally infected pigs. Similarly, the results showed that inguinal lymph node samples, together with serum, spleen and lungs samples, contained more ASFV nucleic acids than the swabs. Our findings demonstrated that the inguinal lymph node collected by minimally invasive sampler is an ideal tissue for diagnosing ASFV infection in dead pigs without necropsy.
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spelling pubmed-95545362022-10-13 Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy Li, Xiaowen Li, Yang Fan, Mingyu Fan, Shiran Gao, Wenchao Ren, Jing Liu, Qingyuan Li, Jingtao Wu, Weisheng Li, Junxian Yu, Qiannan Wang, Xinglong Yan, Zhichun Front Vet Sci Veterinary Science African swine fever (ASF) is a highly contagious hemorrhagic and transboundary animal disease, and it threatens global food security. A full necropsy to harvest the sample matrices for diagnosis in the farm may lead to contamination of the premises and directly threaten to the herds. In the present study, we compared the ASFV loads of the common samples that can be collected without necropsy. The unmatched nasal, throat, rectal samples were randomly taken using cotton swabs, and inguinal lymph node samples were collected by the minimally invasive samplers from the dead pigs of an ASF field outbreak farm. The ASFV loads of the samples were detected by qPCR and the results suggested that the overall ASFV nucleic acids levels of inguinal lymph node samples were higher than the swabs. What's more, sets of matched nasal swabs, rectal swabs, throat swabs, inguinal lymph nodes, serums, spleens and lungs samples were collected from 15 dead ASFV naturally infected pigs. Similarly, the results showed that inguinal lymph node samples, together with serum, spleen and lungs samples, contained more ASFV nucleic acids than the swabs. Our findings demonstrated that the inguinal lymph node collected by minimally invasive sampler is an ideal tissue for diagnosing ASFV infection in dead pigs without necropsy. Frontiers Media S.A. 2022-09-28 /pmc/articles/PMC9554536/ /pubmed/36246330 http://dx.doi.org/10.3389/fvets.2022.1000969 Text en Copyright © 2022 Li, Li, Fan, Fan, Gao, Ren, Liu, Li, Wu, Li, Yu, Wang and Yan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Xiaowen
Li, Yang
Fan, Mingyu
Fan, Shiran
Gao, Wenchao
Ren, Jing
Liu, Qingyuan
Li, Jingtao
Wu, Weisheng
Li, Junxian
Yu, Qiannan
Wang, Xinglong
Yan, Zhichun
Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy
title Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy
title_full Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy
title_fullStr Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy
title_full_unstemmed Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy
title_short Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy
title_sort inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose asf in dead pigs without necropsy
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554536/
https://www.ncbi.nlm.nih.gov/pubmed/36246330
http://dx.doi.org/10.3389/fvets.2022.1000969
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