Cargando…

Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots

Peanut (Arachis hypogaea L.) is an important oil crop globally because of its high edible and economic value. However, its yield and quality are often restricted by certain soil factors, especially nitrogen (N) deficiency, and soil compaction. To explore the molecular mechanisms and metabolic basis...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Liyu, Wu, Qi, Liang, Haiyan, Yin, Liang, Shen, Pu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554563/
https://www.ncbi.nlm.nih.gov/pubmed/36247623
http://dx.doi.org/10.3389/fpls.2022.948742
_version_ 1784806726561693696
author Yang, Liyu
Wu, Qi
Liang, Haiyan
Yin, Liang
Shen, Pu
author_facet Yang, Liyu
Wu, Qi
Liang, Haiyan
Yin, Liang
Shen, Pu
author_sort Yang, Liyu
collection PubMed
description Peanut (Arachis hypogaea L.) is an important oil crop globally because of its high edible and economic value. However, its yield and quality are often restricted by certain soil factors, especially nitrogen (N) deficiency, and soil compaction. To explore the molecular mechanisms and metabolic basis behind the peanut response to N deficiency and soil compaction stresses, transcriptome and metabolome analyses of peanut root were carried out. The results showed that N deficiency and soil compaction stresses clearly impaired the growth and development of peanut's aboveground and underground parts, as well as its root nodulation. A total of 18645 differentially expressed genes (DEGs) and 875 known differentially accumulated metabolites (DAMs) were identified in peanut root under differing soil compaction and N conditions. The transcriptome analysis revealed that DEGs related to N deficiency were mainly enriched in “amino acid metabolism”, “starch and sucrose metabolism”, and “TCA cycle” pathways, while DEGs related to soil compaction were mainly enriched in “oxidoreductase activity”, “lipids metabolism”, and “isoflavonoid biosynthesis” pathways. The metabolome analysis also showed significant differences in the accumulation of metabolisms in these pathways under different stress conditions. Then the involvement of genes and metabolites in pathways of “amino acid metabolism”, “TCA cycle”, “lipids metabolism”, and “isoflavonoid biosynthesis” under different soil compaction and N deficiency stresses were well discussed. This integrated transcriptome and metabolome analysis study enhances our mechanistic knowledge of how peanut plants respond to N deficiency and soil compaction stresses. Moreover, it provides new leads to further investigate candidate functional genes and metabolic pathways for use in improving the adaptability of peanut to abiotic stress and accelerating its breeding process of new stress-resistant varieties.
format Online
Article
Text
id pubmed-9554563
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95545632022-10-13 Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots Yang, Liyu Wu, Qi Liang, Haiyan Yin, Liang Shen, Pu Front Plant Sci Plant Science Peanut (Arachis hypogaea L.) is an important oil crop globally because of its high edible and economic value. However, its yield and quality are often restricted by certain soil factors, especially nitrogen (N) deficiency, and soil compaction. To explore the molecular mechanisms and metabolic basis behind the peanut response to N deficiency and soil compaction stresses, transcriptome and metabolome analyses of peanut root were carried out. The results showed that N deficiency and soil compaction stresses clearly impaired the growth and development of peanut's aboveground and underground parts, as well as its root nodulation. A total of 18645 differentially expressed genes (DEGs) and 875 known differentially accumulated metabolites (DAMs) were identified in peanut root under differing soil compaction and N conditions. The transcriptome analysis revealed that DEGs related to N deficiency were mainly enriched in “amino acid metabolism”, “starch and sucrose metabolism”, and “TCA cycle” pathways, while DEGs related to soil compaction were mainly enriched in “oxidoreductase activity”, “lipids metabolism”, and “isoflavonoid biosynthesis” pathways. The metabolome analysis also showed significant differences in the accumulation of metabolisms in these pathways under different stress conditions. Then the involvement of genes and metabolites in pathways of “amino acid metabolism”, “TCA cycle”, “lipids metabolism”, and “isoflavonoid biosynthesis” under different soil compaction and N deficiency stresses were well discussed. This integrated transcriptome and metabolome analysis study enhances our mechanistic knowledge of how peanut plants respond to N deficiency and soil compaction stresses. Moreover, it provides new leads to further investigate candidate functional genes and metabolic pathways for use in improving the adaptability of peanut to abiotic stress and accelerating its breeding process of new stress-resistant varieties. Frontiers Media S.A. 2022-09-28 /pmc/articles/PMC9554563/ /pubmed/36247623 http://dx.doi.org/10.3389/fpls.2022.948742 Text en Copyright © 2022 Yang, Wu, Liang, Yin and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Liyu
Wu, Qi
Liang, Haiyan
Yin, Liang
Shen, Pu
Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
title Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
title_full Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
title_fullStr Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
title_full_unstemmed Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
title_short Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
title_sort integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9554563/
https://www.ncbi.nlm.nih.gov/pubmed/36247623
http://dx.doi.org/10.3389/fpls.2022.948742
work_keys_str_mv AT yangliyu integratedanalysesoftranscriptomeandmetabolomeprovidesnewinsightsintotheprimaryandsecondarymetabolisminresponsetonitrogendeficiencyandsoilcompactionstressinpeanutroots
AT wuqi integratedanalysesoftranscriptomeandmetabolomeprovidesnewinsightsintotheprimaryandsecondarymetabolisminresponsetonitrogendeficiencyandsoilcompactionstressinpeanutroots
AT lianghaiyan integratedanalysesoftranscriptomeandmetabolomeprovidesnewinsightsintotheprimaryandsecondarymetabolisminresponsetonitrogendeficiencyandsoilcompactionstressinpeanutroots
AT yinliang integratedanalysesoftranscriptomeandmetabolomeprovidesnewinsightsintotheprimaryandsecondarymetabolisminresponsetonitrogendeficiencyandsoilcompactionstressinpeanutroots
AT shenpu integratedanalysesoftranscriptomeandmetabolomeprovidesnewinsightsintotheprimaryandsecondarymetabolisminresponsetonitrogendeficiencyandsoilcompactionstressinpeanutroots