Cargando…

Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells

Bats constitute a large and diverse group of mammals with unique characteristics. One of these is the ability of bats to maintain various pathogens, particularly viruses, without evidence of disease. The innate immune system has been implicated as one of the important components involved in this pro...

Descripción completa

Detalles Bibliográficos
Autores principales: Kuzmin, Ivan V., Ramanathan, Palaniappan, Basler, Christopher F., Bukreyev, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555311/
https://www.ncbi.nlm.nih.gov/pubmed/36237518
http://dx.doi.org/10.3389/fviro.2021.759655
_version_ 1784806875842215936
author Kuzmin, Ivan V.
Ramanathan, Palaniappan
Basler, Christopher F.
Bukreyev, Alexander
author_facet Kuzmin, Ivan V.
Ramanathan, Palaniappan
Basler, Christopher F.
Bukreyev, Alexander
author_sort Kuzmin, Ivan V.
collection PubMed
description Bats constitute a large and diverse group of mammals with unique characteristics. One of these is the ability of bats to maintain various pathogens, particularly viruses, without evidence of disease. The innate immune system has been implicated as one of the important components involved in this process. However, in contrast to the human innate immune system, little data is available for bats. In the present study we generated 23 fusion constructs of innate immune genes of Egyptian fruit bat (Rousettus aegyptiacus) with mCherry as a fluorescent reporter. We evaluated the effects of overexpressing these genes on the replication of Marburg and Ebola viruses in the Egyptian fruit bat cell line R06EJ. Both viruses were substantially inhibited by overexpression of type I, II and III interferons, as well as by DDX58 (RIG-I), IFIH1, and IRF1. Our observations suggest that the broad antiviral activity of these genes reported previously in human cells is conserved in Egyptian fruit bats and these possess anti-filovirus activities that may contribute to the efficient virus clearance.
format Online
Article
Text
id pubmed-9555311
institution National Center for Biotechnology Information
language English
publishDate 2021
record_format MEDLINE/PubMed
spelling pubmed-95553112022-10-12 Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells Kuzmin, Ivan V. Ramanathan, Palaniappan Basler, Christopher F. Bukreyev, Alexander Front Virol Article Bats constitute a large and diverse group of mammals with unique characteristics. One of these is the ability of bats to maintain various pathogens, particularly viruses, without evidence of disease. The innate immune system has been implicated as one of the important components involved in this process. However, in contrast to the human innate immune system, little data is available for bats. In the present study we generated 23 fusion constructs of innate immune genes of Egyptian fruit bat (Rousettus aegyptiacus) with mCherry as a fluorescent reporter. We evaluated the effects of overexpressing these genes on the replication of Marburg and Ebola viruses in the Egyptian fruit bat cell line R06EJ. Both viruses were substantially inhibited by overexpression of type I, II and III interferons, as well as by DDX58 (RIG-I), IFIH1, and IRF1. Our observations suggest that the broad antiviral activity of these genes reported previously in human cells is conserved in Egyptian fruit bats and these possess anti-filovirus activities that may contribute to the efficient virus clearance. 2021-10 2021-10-12 /pmc/articles/PMC9555311/ /pubmed/36237518 http://dx.doi.org/10.3389/fviro.2021.759655 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Article
Kuzmin, Ivan V.
Ramanathan, Palaniappan
Basler, Christopher F.
Bukreyev, Alexander
Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells
title Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells
title_full Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells
title_fullStr Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells
title_full_unstemmed Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells
title_short Effects of Overexpression of the Egyptian Fruit Bat Innate Immune Genes on Filovirus Infections in the Host Cells
title_sort effects of overexpression of the egyptian fruit bat innate immune genes on filovirus infections in the host cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555311/
https://www.ncbi.nlm.nih.gov/pubmed/36237518
http://dx.doi.org/10.3389/fviro.2021.759655
work_keys_str_mv AT kuzminivanv effectsofoverexpressionoftheegyptianfruitbatinnateimmunegenesonfilovirusinfectionsinthehostcells
AT ramanathanpalaniappan effectsofoverexpressionoftheegyptianfruitbatinnateimmunegenesonfilovirusinfectionsinthehostcells
AT baslerchristopherf effectsofoverexpressionoftheegyptianfruitbatinnateimmunegenesonfilovirusinfectionsinthehostcells
AT bukreyevalexander effectsofoverexpressionoftheegyptianfruitbatinnateimmunegenesonfilovirusinfectionsinthehostcells