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Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans
The Musashi family of RNA-binding proteins controls several biological processes including stem cell maintenance, cell division and neural function. Previously, we demonstrated that the C. elegans Musashi ortholog, msi-1, regulates forgetting via translational repression of the Arp2/3 actin-branchin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555661/ https://www.ncbi.nlm.nih.gov/pubmed/36223338 http://dx.doi.org/10.1371/journal.pgen.1010420 |
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author | Mastrandreas, Pavlina Boglari, Csaba Arnold, Andreas Peter, Fabian de Quervain, Dominique J.-F. Papassotiropoulos, Andreas Stetak, Attila |
author_facet | Mastrandreas, Pavlina Boglari, Csaba Arnold, Andreas Peter, Fabian de Quervain, Dominique J.-F. Papassotiropoulos, Andreas Stetak, Attila |
author_sort | Mastrandreas, Pavlina |
collection | PubMed |
description | The Musashi family of RNA-binding proteins controls several biological processes including stem cell maintenance, cell division and neural function. Previously, we demonstrated that the C. elegans Musashi ortholog, msi-1, regulates forgetting via translational repression of the Arp2/3 actin-branching complex. However, the mechanisms controlling MSI-1 activity during the regulation of forgetting are currently unknown. Here we investigated the effects of protein phosphorylation on MSI-1 activity. We showed that MSI-1 function is likely controlled by alterations of its activity rather than its expression levels. Furthermore, we found that MSI-1 is phosphorylated and using mass spectrometry we identified MSI-1 phosphorylation at three residues (T18, S19 and S34). CRISPR-based manipulations of MSI-1 phosphorylation sites revealed that phosphorylation is necessary for MSI-1 function in both short- and long-term aversive olfactory associative memory. Thus, our study provides insight into the mechanisms regulating memory-related MSI-1 activity and may facilitate the development of novel therapeutic approaches. |
format | Online Article Text |
id | pubmed-9555661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95556612022-10-13 Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans Mastrandreas, Pavlina Boglari, Csaba Arnold, Andreas Peter, Fabian de Quervain, Dominique J.-F. Papassotiropoulos, Andreas Stetak, Attila PLoS Genet Research Article The Musashi family of RNA-binding proteins controls several biological processes including stem cell maintenance, cell division and neural function. Previously, we demonstrated that the C. elegans Musashi ortholog, msi-1, regulates forgetting via translational repression of the Arp2/3 actin-branching complex. However, the mechanisms controlling MSI-1 activity during the regulation of forgetting are currently unknown. Here we investigated the effects of protein phosphorylation on MSI-1 activity. We showed that MSI-1 function is likely controlled by alterations of its activity rather than its expression levels. Furthermore, we found that MSI-1 is phosphorylated and using mass spectrometry we identified MSI-1 phosphorylation at three residues (T18, S19 and S34). CRISPR-based manipulations of MSI-1 phosphorylation sites revealed that phosphorylation is necessary for MSI-1 function in both short- and long-term aversive olfactory associative memory. Thus, our study provides insight into the mechanisms regulating memory-related MSI-1 activity and may facilitate the development of novel therapeutic approaches. Public Library of Science 2022-10-12 /pmc/articles/PMC9555661/ /pubmed/36223338 http://dx.doi.org/10.1371/journal.pgen.1010420 Text en © 2022 Mastrandreas et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mastrandreas, Pavlina Boglari, Csaba Arnold, Andreas Peter, Fabian de Quervain, Dominique J.-F. Papassotiropoulos, Andreas Stetak, Attila Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans |
title | Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans |
title_full | Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans |
title_fullStr | Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans |
title_full_unstemmed | Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans |
title_short | Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans |
title_sort | phosphorylation of msi-1 is implicated in the regulation of associative memory in caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555661/ https://www.ncbi.nlm.nih.gov/pubmed/36223338 http://dx.doi.org/10.1371/journal.pgen.1010420 |
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