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Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors
The prefrontal cortex (PFC) is a hub for cognitive control, and dopamine profoundly influences its functions. In other brain regions, astrocytes sense diverse neurotransmitters and neuromodulators and, in turn, orchestrate regulation of neuroactive substances. However, basic physiology of PFC astroc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555850/ https://www.ncbi.nlm.nih.gov/pubmed/36170823 http://dx.doi.org/10.1016/j.celrep.2022.111426 |
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author | Pittolo, Silvia Yokoyama, Sae Willoughby, Drew D. Taylor, Charlotte R. Reitman, Michael E. Tse, Vincent Wu, Zhaofa Etchenique, Roberto Li, Yulong Poskanzer, Kira E. |
author_facet | Pittolo, Silvia Yokoyama, Sae Willoughby, Drew D. Taylor, Charlotte R. Reitman, Michael E. Tse, Vincent Wu, Zhaofa Etchenique, Roberto Li, Yulong Poskanzer, Kira E. |
author_sort | Pittolo, Silvia |
collection | PubMed |
description | The prefrontal cortex (PFC) is a hub for cognitive control, and dopamine profoundly influences its functions. In other brain regions, astrocytes sense diverse neurotransmitters and neuromodulators and, in turn, orchestrate regulation of neuroactive substances. However, basic physiology of PFC astrocytes, including which neuromodulatory signals they respond to and how they contribute to PFC function, is unclear. Here, we characterize divergent signaling signatures in mouse astrocytes of the PFC and primary sensory cortex, which show differential responsiveness to locomotion. We find that PFC astrocytes express receptors for dopamine but are unresponsive through the Gs/Gi-cAMP pathway. Instead, fast calcium signals in PFC astrocytes are time locked to dopamine release and are mediated by α1-adrenergic receptors both ex vivo and in vivo. Further, we describe dopamine-triggered regulation of extracellular ATP at PFC astrocyte territories. Thus, we identify astrocytes as active players in dopaminergic signaling in the PFC, contributing to PFC function though neuromodulator receptor crosstalk. |
format | Online Article Text |
id | pubmed-9555850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
record_format | MEDLINE/PubMed |
spelling | pubmed-95558502022-10-12 Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors Pittolo, Silvia Yokoyama, Sae Willoughby, Drew D. Taylor, Charlotte R. Reitman, Michael E. Tse, Vincent Wu, Zhaofa Etchenique, Roberto Li, Yulong Poskanzer, Kira E. Cell Rep Article The prefrontal cortex (PFC) is a hub for cognitive control, and dopamine profoundly influences its functions. In other brain regions, astrocytes sense diverse neurotransmitters and neuromodulators and, in turn, orchestrate regulation of neuroactive substances. However, basic physiology of PFC astrocytes, including which neuromodulatory signals they respond to and how they contribute to PFC function, is unclear. Here, we characterize divergent signaling signatures in mouse astrocytes of the PFC and primary sensory cortex, which show differential responsiveness to locomotion. We find that PFC astrocytes express receptors for dopamine but are unresponsive through the Gs/Gi-cAMP pathway. Instead, fast calcium signals in PFC astrocytes are time locked to dopamine release and are mediated by α1-adrenergic receptors both ex vivo and in vivo. Further, we describe dopamine-triggered regulation of extracellular ATP at PFC astrocyte territories. Thus, we identify astrocytes as active players in dopaminergic signaling in the PFC, contributing to PFC function though neuromodulator receptor crosstalk. 2022-09-27 /pmc/articles/PMC9555850/ /pubmed/36170823 http://dx.doi.org/10.1016/j.celrep.2022.111426 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Pittolo, Silvia Yokoyama, Sae Willoughby, Drew D. Taylor, Charlotte R. Reitman, Michael E. Tse, Vincent Wu, Zhaofa Etchenique, Roberto Li, Yulong Poskanzer, Kira E. Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
title | Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
title_full | Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
title_fullStr | Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
title_full_unstemmed | Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
title_short | Dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
title_sort | dopamine activates astrocytes in prefrontal cortex via α1-adrenergic receptors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9555850/ https://www.ncbi.nlm.nih.gov/pubmed/36170823 http://dx.doi.org/10.1016/j.celrep.2022.111426 |
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