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Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India
Platyhelminths belonging to the family Clinostomidae (Digenea) have a worldwide distribution and are known to infect piscivorous birds through their intermediate hosts, usually fish species. In the present study, clinostome metacercariae were collected from fish hosts, including Channa punctata (Blo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556428/ https://www.ncbi.nlm.nih.gov/pubmed/36070023 http://dx.doi.org/10.1007/s00436-022-07644-y |
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author | Choudhary, Kirti Ray, Shailendra Shamsi, Shokoofeh Agrawal, Nirupama |
author_facet | Choudhary, Kirti Ray, Shailendra Shamsi, Shokoofeh Agrawal, Nirupama |
author_sort | Choudhary, Kirti |
collection | PubMed |
description | Platyhelminths belonging to the family Clinostomidae (Digenea) have a worldwide distribution and are known to infect piscivorous birds through their intermediate hosts, usually fish species. In the present study, clinostome metacercariae were collected from fish hosts, including Channa punctata (Bloch 1793) (n = 25) and Trichogaster fasciata Bloch and Schneider 1801 (n = 25), from a freshwater system in India. The experimental infection of cattle egrets, Ardea (Bubulcus) ibis Linnaeus 1758, with some of the live metacercariae found in the present study was successful. Live adult parasites were obtained from the buccal cavity of the birds. Both metacercaria and adult specimens were subjected to molecular studies to obtain the sequences of 28S, ITS1, and ITS2 (nuclear rDNA) regions. The parasites were found to belong to three species, Clinostomum giganticum Agarwal 1959; C. piscidium Southwell and Prashad 1918; and Euclinostomum heterostomum (Rudolphi 1809). Phylogenetic analyses of the sequences obtained from the adults and metacercariae established a link between the metacercariae in the fish hosts and adults in the avian host, which is essential to elucidate their partial life cycle and specify morphological characteristics in the metacercarial stage. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00436-022-07644-y. |
format | Online Article Text |
id | pubmed-9556428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-95564282022-10-14 Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India Choudhary, Kirti Ray, Shailendra Shamsi, Shokoofeh Agrawal, Nirupama Parasitol Res Fish Parasitology - Original Paper Platyhelminths belonging to the family Clinostomidae (Digenea) have a worldwide distribution and are known to infect piscivorous birds through their intermediate hosts, usually fish species. In the present study, clinostome metacercariae were collected from fish hosts, including Channa punctata (Bloch 1793) (n = 25) and Trichogaster fasciata Bloch and Schneider 1801 (n = 25), from a freshwater system in India. The experimental infection of cattle egrets, Ardea (Bubulcus) ibis Linnaeus 1758, with some of the live metacercariae found in the present study was successful. Live adult parasites were obtained from the buccal cavity of the birds. Both metacercaria and adult specimens were subjected to molecular studies to obtain the sequences of 28S, ITS1, and ITS2 (nuclear rDNA) regions. The parasites were found to belong to three species, Clinostomum giganticum Agarwal 1959; C. piscidium Southwell and Prashad 1918; and Euclinostomum heterostomum (Rudolphi 1809). Phylogenetic analyses of the sequences obtained from the adults and metacercariae established a link between the metacercariae in the fish hosts and adults in the avian host, which is essential to elucidate their partial life cycle and specify morphological characteristics in the metacercarial stage. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00436-022-07644-y. Springer Berlin Heidelberg 2022-09-07 2022 /pmc/articles/PMC9556428/ /pubmed/36070023 http://dx.doi.org/10.1007/s00436-022-07644-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Fish Parasitology - Original Paper Choudhary, Kirti Ray, Shailendra Shamsi, Shokoofeh Agrawal, Nirupama Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India |
title | Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India |
title_full | Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India |
title_fullStr | Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India |
title_full_unstemmed | Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India |
title_short | Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India |
title_sort | characterization of clinostomum (digenea: clinostomidae) spp. in india |
topic | Fish Parasitology - Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556428/ https://www.ncbi.nlm.nih.gov/pubmed/36070023 http://dx.doi.org/10.1007/s00436-022-07644-y |
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