Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization

In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase ac...

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Detalles Bibliográficos
Autores principales: López-Sanz, Celia, Sánchez-Martínez, Elisa, Jiménez-Saiz, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556792/
https://www.ncbi.nlm.nih.gov/pubmed/36223269
http://dx.doi.org/10.1016/j.xpro.2022.101755
Descripción
Sumario:In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches. For complete details on the use and execution of this protocol, please refer to López-Sanz et al. (2022).

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