Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization

In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase ac...

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Autores principales: López-Sanz, Celia, Sánchez-Martínez, Elisa, Jiménez-Saiz, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556792/
https://www.ncbi.nlm.nih.gov/pubmed/36223269
http://dx.doi.org/10.1016/j.xpro.2022.101755
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author López-Sanz, Celia
Sánchez-Martínez, Elisa
Jiménez-Saiz, Rodrigo
author_facet López-Sanz, Celia
Sánchez-Martínez, Elisa
Jiménez-Saiz, Rodrigo
author_sort López-Sanz, Celia
collection PubMed
description In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches. For complete details on the use and execution of this protocol, please refer to López-Sanz et al. (2022).
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spelling pubmed-95567922022-10-14 Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization López-Sanz, Celia Sánchez-Martínez, Elisa Jiménez-Saiz, Rodrigo STAR Protoc Protocol In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches. For complete details on the use and execution of this protocol, please refer to López-Sanz et al. (2022). Elsevier 2022-10-10 /pmc/articles/PMC9556792/ /pubmed/36223269 http://dx.doi.org/10.1016/j.xpro.2022.101755 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
López-Sanz, Celia
Sánchez-Martínez, Elisa
Jiménez-Saiz, Rodrigo
Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
title Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
title_full Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
title_fullStr Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
title_full_unstemmed Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
title_short Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
title_sort protocol to desensitize human and murine mast cells after polyclonal ige sensitization
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556792/
https://www.ncbi.nlm.nih.gov/pubmed/36223269
http://dx.doi.org/10.1016/j.xpro.2022.101755
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