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Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase ac...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556792/ https://www.ncbi.nlm.nih.gov/pubmed/36223269 http://dx.doi.org/10.1016/j.xpro.2022.101755 |