Cargando…

Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells

Cinquefoils have been widely used in local folk medicine in Europe and Asia to manage various gastrointestinal inflammations and/or infections, certain forms of cancer, thyroid gland disorders, and wound healing. In the present paper, acetone extracts from aerial parts of selected Potentilla species...

Descripción completa

Detalles Bibliográficos
Autores principales: Augustynowicz, Daniel, Lemieszek, Marta Kinga, Strawa, Jakub Władysław, Wiater, Adrian, Tomczyk, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556846/
https://www.ncbi.nlm.nih.gov/pubmed/36249795
http://dx.doi.org/10.3389/fphar.2022.1027315
_version_ 1784807164732243968
author Augustynowicz, Daniel
Lemieszek, Marta Kinga
Strawa, Jakub Władysław
Wiater, Adrian
Tomczyk, Michał
author_facet Augustynowicz, Daniel
Lemieszek, Marta Kinga
Strawa, Jakub Władysław
Wiater, Adrian
Tomczyk, Michał
author_sort Augustynowicz, Daniel
collection PubMed
description Cinquefoils have been widely used in local folk medicine in Europe and Asia to manage various gastrointestinal inflammations and/or infections, certain forms of cancer, thyroid gland disorders, and wound healing. In the present paper, acetone extracts from aerial parts of selected Potentilla species, namely P. alba (PAL7), P. argentea (PAR7), P. grandiflora (PGR7), P. norvegica (PN7), P. recta (PRE7), and the closely related Drymocalis rupestris (syn. P. rupestris) (PRU7), were analysed for their cytotoxicity and antiproliferative activities against human colon adenocarcinoma cell line LS180 and human colon epithelial cell line CCD841 CoN. Moreover, quantitative assessments of the total polyphenolic (TPC), total tannin (TTC), total proanthocyanidins (TPrC), total flavonoid (TFC), and total phenolic acid (TPAC) were conducted. The analysis of secondary metabolite composition was carried out by LC-PDA-HRMS. The highest TPC and TTC were found in PAR7 (339.72 and 246.92 mg gallic acid equivalents (GAE)/g extract, respectively) and PN7 (332.11 and 252.3 mg GAE/g extract, respectively). The highest TPrC, TFC, and TPAC levels were found for PAL7 (21.28 mg catechin equivalents (CAT)/g extract, 71.85 mg rutin equivalents (RE)/g extract, and 124.18 mg caffeic acid equivalents (CAE)/g extract, respectively). LC-PDA-HRMS analysis revealed the presence of 83 compounds, including brevifolincarboxylic acid, ellagic acid, pedunculagin, agrimoniin, chlorogenic acid, astragalin, and tiliroside. Moreover, the presence of tri-coumaroyl spermidine was demonstrated for the first time in the genus Potentilla. Results of the MTT assay revealed that all tested extracts decreased the viability of both cell lines; however, a markedly stronger effect was observed in the colon cancer cells. The highest selectivity was demonstrated by PAR7, which effectively inhibited the metabolic activity of LS180 cells (IC(50) = 38 μg/ml), while at the same time causing the lowest unwanted effects in CCD841 CoN cells (IC(50) = 1,134 μg/ml). BrdU assay revealed a significant decrease in DNA synthesis in both examined cell lines in response to all investigated extracts. It should be emphasized that the tested extracts had a stronger effect on colon cancer cells than normal colon cells, and the most significant antiproliferative properties were observed in the case of PAR7 (IC(50) LS180 = 174 μg/ml) and PN7 (IC(50) LS180 = 169 μg/ml). The results of LDH assay revealed that all tested extracts were not cytotoxic against normal colon epithelial cells, whereas in the cancer cells, all compounds significantly damaged cell membranes, and the observed effect was dose-dependent. The highest cytotoxicity was observed in LS180 cells in response to PAR7, which, in concentrations ranging from 25 to 250 μg/ml, increased LDH release by 110%–1,062%, respectively. Performed studies have revealed that all Potentilla species may be useful sources for anti-colorectal cancer agents; however, additional research is required to prove this definitively.
format Online
Article
Text
id pubmed-9556846
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95568462022-10-14 Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells Augustynowicz, Daniel Lemieszek, Marta Kinga Strawa, Jakub Władysław Wiater, Adrian Tomczyk, Michał Front Pharmacol Pharmacology Cinquefoils have been widely used in local folk medicine in Europe and Asia to manage various gastrointestinal inflammations and/or infections, certain forms of cancer, thyroid gland disorders, and wound healing. In the present paper, acetone extracts from aerial parts of selected Potentilla species, namely P. alba (PAL7), P. argentea (PAR7), P. grandiflora (PGR7), P. norvegica (PN7), P. recta (PRE7), and the closely related Drymocalis rupestris (syn. P. rupestris) (PRU7), were analysed for their cytotoxicity and antiproliferative activities against human colon adenocarcinoma cell line LS180 and human colon epithelial cell line CCD841 CoN. Moreover, quantitative assessments of the total polyphenolic (TPC), total tannin (TTC), total proanthocyanidins (TPrC), total flavonoid (TFC), and total phenolic acid (TPAC) were conducted. The analysis of secondary metabolite composition was carried out by LC-PDA-HRMS. The highest TPC and TTC were found in PAR7 (339.72 and 246.92 mg gallic acid equivalents (GAE)/g extract, respectively) and PN7 (332.11 and 252.3 mg GAE/g extract, respectively). The highest TPrC, TFC, and TPAC levels were found for PAL7 (21.28 mg catechin equivalents (CAT)/g extract, 71.85 mg rutin equivalents (RE)/g extract, and 124.18 mg caffeic acid equivalents (CAE)/g extract, respectively). LC-PDA-HRMS analysis revealed the presence of 83 compounds, including brevifolincarboxylic acid, ellagic acid, pedunculagin, agrimoniin, chlorogenic acid, astragalin, and tiliroside. Moreover, the presence of tri-coumaroyl spermidine was demonstrated for the first time in the genus Potentilla. Results of the MTT assay revealed that all tested extracts decreased the viability of both cell lines; however, a markedly stronger effect was observed in the colon cancer cells. The highest selectivity was demonstrated by PAR7, which effectively inhibited the metabolic activity of LS180 cells (IC(50) = 38 μg/ml), while at the same time causing the lowest unwanted effects in CCD841 CoN cells (IC(50) = 1,134 μg/ml). BrdU assay revealed a significant decrease in DNA synthesis in both examined cell lines in response to all investigated extracts. It should be emphasized that the tested extracts had a stronger effect on colon cancer cells than normal colon cells, and the most significant antiproliferative properties were observed in the case of PAR7 (IC(50) LS180 = 174 μg/ml) and PN7 (IC(50) LS180 = 169 μg/ml). The results of LDH assay revealed that all tested extracts were not cytotoxic against normal colon epithelial cells, whereas in the cancer cells, all compounds significantly damaged cell membranes, and the observed effect was dose-dependent. The highest cytotoxicity was observed in LS180 cells in response to PAR7, which, in concentrations ranging from 25 to 250 μg/ml, increased LDH release by 110%–1,062%, respectively. Performed studies have revealed that all Potentilla species may be useful sources for anti-colorectal cancer agents; however, additional research is required to prove this definitively. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9556846/ /pubmed/36249795 http://dx.doi.org/10.3389/fphar.2022.1027315 Text en Copyright © 2022 Augustynowicz, Lemieszek, Strawa, Wiater and Tomczyk. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Augustynowicz, Daniel
Lemieszek, Marta Kinga
Strawa, Jakub Władysław
Wiater, Adrian
Tomczyk, Michał
Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells
title Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells
title_full Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells
title_fullStr Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells
title_full_unstemmed Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells
title_short Anticancer potential of acetone extracts from selected Potentilla species against human colorectal cancer cells
title_sort anticancer potential of acetone extracts from selected potentilla species against human colorectal cancer cells
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556846/
https://www.ncbi.nlm.nih.gov/pubmed/36249795
http://dx.doi.org/10.3389/fphar.2022.1027315
work_keys_str_mv AT augustynowiczdaniel anticancerpotentialofacetoneextractsfromselectedpotentillaspeciesagainsthumancolorectalcancercells
AT lemieszekmartakinga anticancerpotentialofacetoneextractsfromselectedpotentillaspeciesagainsthumancolorectalcancercells
AT strawajakubwładysław anticancerpotentialofacetoneextractsfromselectedpotentillaspeciesagainsthumancolorectalcancercells
AT wiateradrian anticancerpotentialofacetoneextractsfromselectedpotentillaspeciesagainsthumancolorectalcancercells
AT tomczykmichał anticancerpotentialofacetoneextractsfromselectedpotentillaspeciesagainsthumancolorectalcancercells