Cargando…

Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro

Culturing mesenchymal stromal cells (MSC) in human platelet lysate (HPL) supplemented media can enhance their osteogenic differentiation potential. The objective of this study was to test the hypothesis that conditioned media (CM) derived from HPL-cultured MSC also have pro-osteogenic effects. Poole...

Descripción completa

Detalles Bibliográficos
Autores principales: Shanbhag, Siddharth, Al-Sharabi, Niyaz, Mohamed-Ahmed, Samih, Gruber, Reinhard, Kristoffersen, Einar K., Mustafa, Kamal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556861/
https://www.ncbi.nlm.nih.gov/pubmed/36246352
http://dx.doi.org/10.3389/fbioe.2022.969275
_version_ 1784807168787087360
author Shanbhag, Siddharth
Al-Sharabi, Niyaz
Mohamed-Ahmed, Samih
Gruber, Reinhard
Kristoffersen, Einar K.
Mustafa, Kamal
author_facet Shanbhag, Siddharth
Al-Sharabi, Niyaz
Mohamed-Ahmed, Samih
Gruber, Reinhard
Kristoffersen, Einar K.
Mustafa, Kamal
author_sort Shanbhag, Siddharth
collection PubMed
description Culturing mesenchymal stromal cells (MSC) in human platelet lysate (HPL) supplemented media can enhance their osteogenic differentiation potential. The objective of this study was to test the hypothesis that conditioned media (CM) derived from HPL-cultured MSC also have pro-osteogenic effects. Pooled CM was prepared from HPL-cultured human bone marrow MSC (BMSC) of multiple donors and applied on BMSC of different donors (than those used for CM preparation), with or without additional supplementation [HPL, fetal bovine serum (FBS)] and osteogenic stimulation. At various time-points, cell proliferation, alkaline phosphatase (ALP) activity, osteogenic gene expression and in vitro mineralization were assessed. BMSC in standard unstimulated growth media served as controls. After 3–7 days, CM alone did not promote BMSC proliferation or ALP activity; supplementation of CM with HPL slightly improved these effects. After 2 and 7 days, CM alone, but not CM supplemented with HPL, promoted osteogenic gene expression. After 14 days, only CM supplemented with FBS and osteogenic stimulants supported in vitro BMSC mineralization; CM alone and CM supplemented with HPL did not support mineralization, regardless of osteogenic stimulation. In summary, CM from HPL-cultured BMSC promoted osteogenic gene expression but not in vitro mineralization in allogeneic BMSC even when supplemented with HPL and/or osteogenic stimulants. Future studies should investigate the role and relevance of supplementation and osteogenic induction in in vitro assays using CM from MSC.
format Online
Article
Text
id pubmed-9556861
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95568612022-10-14 Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro Shanbhag, Siddharth Al-Sharabi, Niyaz Mohamed-Ahmed, Samih Gruber, Reinhard Kristoffersen, Einar K. Mustafa, Kamal Front Bioeng Biotechnol Bioengineering and Biotechnology Culturing mesenchymal stromal cells (MSC) in human platelet lysate (HPL) supplemented media can enhance their osteogenic differentiation potential. The objective of this study was to test the hypothesis that conditioned media (CM) derived from HPL-cultured MSC also have pro-osteogenic effects. Pooled CM was prepared from HPL-cultured human bone marrow MSC (BMSC) of multiple donors and applied on BMSC of different donors (than those used for CM preparation), with or without additional supplementation [HPL, fetal bovine serum (FBS)] and osteogenic stimulation. At various time-points, cell proliferation, alkaline phosphatase (ALP) activity, osteogenic gene expression and in vitro mineralization were assessed. BMSC in standard unstimulated growth media served as controls. After 3–7 days, CM alone did not promote BMSC proliferation or ALP activity; supplementation of CM with HPL slightly improved these effects. After 2 and 7 days, CM alone, but not CM supplemented with HPL, promoted osteogenic gene expression. After 14 days, only CM supplemented with FBS and osteogenic stimulants supported in vitro BMSC mineralization; CM alone and CM supplemented with HPL did not support mineralization, regardless of osteogenic stimulation. In summary, CM from HPL-cultured BMSC promoted osteogenic gene expression but not in vitro mineralization in allogeneic BMSC even when supplemented with HPL and/or osteogenic stimulants. Future studies should investigate the role and relevance of supplementation and osteogenic induction in in vitro assays using CM from MSC. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9556861/ /pubmed/36246352 http://dx.doi.org/10.3389/fbioe.2022.969275 Text en Copyright © 2022 Shanbhag, Al-Sharabi, Mohamed-Ahmed, Gruber, Kristoffersen and Mustafa. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Shanbhag, Siddharth
Al-Sharabi, Niyaz
Mohamed-Ahmed, Samih
Gruber, Reinhard
Kristoffersen, Einar K.
Mustafa, Kamal
Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
title Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
title_full Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
title_fullStr Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
title_full_unstemmed Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
title_short Brief communication: Effects of conditioned media from human platelet lysate cultured MSC on osteogenic cell differentiation in vitro
title_sort brief communication: effects of conditioned media from human platelet lysate cultured msc on osteogenic cell differentiation in vitro
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556861/
https://www.ncbi.nlm.nih.gov/pubmed/36246352
http://dx.doi.org/10.3389/fbioe.2022.969275
work_keys_str_mv AT shanbhagsiddharth briefcommunicationeffectsofconditionedmediafromhumanplateletlysateculturedmsconosteogeniccelldifferentiationinvitro
AT alsharabiniyaz briefcommunicationeffectsofconditionedmediafromhumanplateletlysateculturedmsconosteogeniccelldifferentiationinvitro
AT mohamedahmedsamih briefcommunicationeffectsofconditionedmediafromhumanplateletlysateculturedmsconosteogeniccelldifferentiationinvitro
AT gruberreinhard briefcommunicationeffectsofconditionedmediafromhumanplateletlysateculturedmsconosteogeniccelldifferentiationinvitro
AT kristofferseneinark briefcommunicationeffectsofconditionedmediafromhumanplateletlysateculturedmsconosteogeniccelldifferentiationinvitro
AT mustafakamal briefcommunicationeffectsofconditionedmediafromhumanplateletlysateculturedmsconosteogeniccelldifferentiationinvitro