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CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda

Insulin-like peptides (ILPs) play key roles in animal growth, metabolism and reproduction in vertebrates. In crustaceans, one type of ILPs, insulin-like androgenic gland hormone (IAG) had been reported to be related to the sex differentiations. However, the function of other types of ILPs is rarely...

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Autores principales: Gao, Yi, Zhang, Xiaojun, Yuan, Jianbo, Zhang, Chengsong, Li, Shihao, Li, Fuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556898/
https://www.ncbi.nlm.nih.gov/pubmed/36246877
http://dx.doi.org/10.3389/fendo.2022.986491
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author Gao, Yi
Zhang, Xiaojun
Yuan, Jianbo
Zhang, Chengsong
Li, Shihao
Li, Fuhua
author_facet Gao, Yi
Zhang, Xiaojun
Yuan, Jianbo
Zhang, Chengsong
Li, Shihao
Li, Fuhua
author_sort Gao, Yi
collection PubMed
description Insulin-like peptides (ILPs) play key roles in animal growth, metabolism and reproduction in vertebrates. In crustaceans, one type of ILPs, insulin-like androgenic gland hormone (IAG) had been reported to be related to the sex differentiations. However, the function of other types of ILPs is rarely reported. Here, we identified another type of ILPs in the ridgetail white prawn Exopalaemon carinicauda (EcILP), which is an ortholog of Drosophila melanogaster ILP7. Sequence characterization and expression analyses showed that EcILP is similar to vertebrate insulin/IGFs and insect ILPs in its heterodimeric structure and expression profile. Using CRISPR/Cas9 genome editing technology, we generated EcILP knockout (KO) prawns. EcILP-KO individuals have a significant higher growth-inhibitory trait and mortality than those in the normal group. In addition, knockdown of EcILP by RNA interference (RNAi) resulted in slower growth rate and higher mortality. These results indicated that EcILP was an important growth regulator in E. carinicauda.
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spelling pubmed-95568982022-10-14 CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda Gao, Yi Zhang, Xiaojun Yuan, Jianbo Zhang, Chengsong Li, Shihao Li, Fuhua Front Endocrinol (Lausanne) Endocrinology Insulin-like peptides (ILPs) play key roles in animal growth, metabolism and reproduction in vertebrates. In crustaceans, one type of ILPs, insulin-like androgenic gland hormone (IAG) had been reported to be related to the sex differentiations. However, the function of other types of ILPs is rarely reported. Here, we identified another type of ILPs in the ridgetail white prawn Exopalaemon carinicauda (EcILP), which is an ortholog of Drosophila melanogaster ILP7. Sequence characterization and expression analyses showed that EcILP is similar to vertebrate insulin/IGFs and insect ILPs in its heterodimeric structure and expression profile. Using CRISPR/Cas9 genome editing technology, we generated EcILP knockout (KO) prawns. EcILP-KO individuals have a significant higher growth-inhibitory trait and mortality than those in the normal group. In addition, knockdown of EcILP by RNA interference (RNAi) resulted in slower growth rate and higher mortality. These results indicated that EcILP was an important growth regulator in E. carinicauda. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9556898/ /pubmed/36246877 http://dx.doi.org/10.3389/fendo.2022.986491 Text en Copyright © 2022 Gao, Zhang, Yuan, Zhang, Li and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Gao, Yi
Zhang, Xiaojun
Yuan, Jianbo
Zhang, Chengsong
Li, Shihao
Li, Fuhua
CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda
title CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda
title_full CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda
title_fullStr CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda
title_full_unstemmed CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda
title_short CRISPR/Cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn Exopalaemon carinicauda
title_sort crispr/cas9-mediated mutation on an insulin-like peptide encoding gene affects the growth of the ridgetail white prawn exopalaemon carinicauda
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556898/
https://www.ncbi.nlm.nih.gov/pubmed/36246877
http://dx.doi.org/10.3389/fendo.2022.986491
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