Cargando…
Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis
Genetic predisposition and disruption of host gut microbiota and immune system can result in inflammatory bowel disease (IBD). Here, we show that miRNA-149-5p (miR-149-5p) and miRNA-149-3p (miR-149-3p) play crucial roles in IBD. Mice lacking miR-149-3p were considerably more susceptible to dextran s...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556934/ https://www.ncbi.nlm.nih.gov/pubmed/36250208 http://dx.doi.org/10.1016/j.omtn.2022.09.018 |
_version_ | 1784807186855100416 |
---|---|
author | Feng, Qingqing Li, Yuanqiang Zhang, Hongli Wang, Ziwei Nie, Xiaobo Yao, Denglin Han, Lu Chen, Wei-Dong Wang, Yan-Dong |
author_facet | Feng, Qingqing Li, Yuanqiang Zhang, Hongli Wang, Ziwei Nie, Xiaobo Yao, Denglin Han, Lu Chen, Wei-Dong Wang, Yan-Dong |
author_sort | Feng, Qingqing |
collection | PubMed |
description | Genetic predisposition and disruption of host gut microbiota and immune system can result in inflammatory bowel disease (IBD). Here, we show that miRNA-149-5p (miR-149-5p) and miRNA-149-3p (miR-149-3p) play crucial roles in IBD. Mice lacking miR-149-3p were considerably more susceptible to dextran sulfate sodium (DSS)-induced colitis than wild-type (WT) mice, accompanied by more serious inflammatory symptoms and increased gene expression of certain inflammatory cytokines. Both miR-149-5p and miR-149-3p suppressed colon inflammatory response in vitro and in vivo. Furthermore, we found significant differences in the composition of the gut microbiota between WT and miR-149-3p(−/−) mice by 16S rRNA sequencing. Co-housing endowed susceptibility to WT mice against DSS-induced colitis compared with the WT control group. However, susceptibility of miR-149-3p(−/−) mice against DSS-induced colitis was still present after antibiotic treatment. These findings suggest that the deletion of miR-149-3p altered gut microbiota and influenced pathogenesis of intestinal inflammation, but sensitivity of miR-149-3p(−/−) mice to DSS-induced colitis is not conferred by microbiota. In addition, we identified the roles of miR-149-5p and miR-149-3p in colon inflammation, which may serve as an attractive therapeutic tool for colitis or IBD, and even colitis-associated carcinoma. |
format | Online Article Text |
id | pubmed-9556934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-95569342022-10-14 Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis Feng, Qingqing Li, Yuanqiang Zhang, Hongli Wang, Ziwei Nie, Xiaobo Yao, Denglin Han, Lu Chen, Wei-Dong Wang, Yan-Dong Mol Ther Nucleic Acids Original Article Genetic predisposition and disruption of host gut microbiota and immune system can result in inflammatory bowel disease (IBD). Here, we show that miRNA-149-5p (miR-149-5p) and miRNA-149-3p (miR-149-3p) play crucial roles in IBD. Mice lacking miR-149-3p were considerably more susceptible to dextran sulfate sodium (DSS)-induced colitis than wild-type (WT) mice, accompanied by more serious inflammatory symptoms and increased gene expression of certain inflammatory cytokines. Both miR-149-5p and miR-149-3p suppressed colon inflammatory response in vitro and in vivo. Furthermore, we found significant differences in the composition of the gut microbiota between WT and miR-149-3p(−/−) mice by 16S rRNA sequencing. Co-housing endowed susceptibility to WT mice against DSS-induced colitis compared with the WT control group. However, susceptibility of miR-149-3p(−/−) mice against DSS-induced colitis was still present after antibiotic treatment. These findings suggest that the deletion of miR-149-3p altered gut microbiota and influenced pathogenesis of intestinal inflammation, but sensitivity of miR-149-3p(−/−) mice to DSS-induced colitis is not conferred by microbiota. In addition, we identified the roles of miR-149-5p and miR-149-3p in colon inflammation, which may serve as an attractive therapeutic tool for colitis or IBD, and even colitis-associated carcinoma. American Society of Gene & Cell Therapy 2022-09-24 /pmc/articles/PMC9556934/ /pubmed/36250208 http://dx.doi.org/10.1016/j.omtn.2022.09.018 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Feng, Qingqing Li, Yuanqiang Zhang, Hongli Wang, Ziwei Nie, Xiaobo Yao, Denglin Han, Lu Chen, Wei-Dong Wang, Yan-Dong Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
title | Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
title_full | Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
title_fullStr | Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
title_full_unstemmed | Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
title_short | Deficiency of miRNA-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
title_sort | deficiency of mirna-149-3p shaped gut microbiota and enhanced dextran sulfate sodium-induced colitis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556934/ https://www.ncbi.nlm.nih.gov/pubmed/36250208 http://dx.doi.org/10.1016/j.omtn.2022.09.018 |
work_keys_str_mv | AT fengqingqing deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT liyuanqiang deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT zhanghongli deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT wangziwei deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT niexiaobo deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT yaodenglin deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT hanlu deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT chenweidong deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis AT wangyandong deficiencyofmirna1493pshapedgutmicrobiotaandenhanceddextransulfatesodiuminducedcolitis |