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Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens

Mycoplasma gallisepticum (M. gallisepticum) is a primary respiratory pathogen of poultry and causes significant economic losses to the poultry industry. There were no reported articles concerning the Pharmacokinetics/Pharmacodynamics (PK/PD) interactions of tilmicosin against M. gallisepticum in viv...

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Autores principales: Zhang, Nan, Zhou, Minghu, Yan, Xiu, Liu, Jinxin, Yuan, Sheng, Yang, Hong, Ding, Huanzhong, Zhang, Dexian, Bai, Yinshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557078/
https://www.ncbi.nlm.nih.gov/pubmed/36246335
http://dx.doi.org/10.3389/fvets.2022.952599
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author Zhang, Nan
Zhou, Minghu
Yan, Xiu
Liu, Jinxin
Yuan, Sheng
Yang, Hong
Ding, Huanzhong
Zhang, Dexian
Bai, Yinshan
author_facet Zhang, Nan
Zhou, Minghu
Yan, Xiu
Liu, Jinxin
Yuan, Sheng
Yang, Hong
Ding, Huanzhong
Zhang, Dexian
Bai, Yinshan
author_sort Zhang, Nan
collection PubMed
description Mycoplasma gallisepticum (M. gallisepticum) is a primary respiratory pathogen of poultry and causes significant economic losses to the poultry industry. There were no reported articles concerning the Pharmacokinetics/Pharmacodynamics (PK/PD) interactions of tilmicosin against M. gallisepticum in vivo. In the current study, we established an in vivo M. gallisepticum infection model and tilmicosin was administered orally to the M. gallisepticum-infected chickens by different dosage regimens. The concentration of tilmicosin in lung tissue was determined by high-pressure liquid chromatography/tandem mass spectrometry (HPLC–MS/MS), besides the counting of the viable colony of M. gallisepticum in lung tissue was also monitored dynamically to appraise the PK/PD interactions of tilmicosin against M. gallisepticum. We found that anti-mycoplasmal activity was concentration-dependent and mycoplasmacidal activity was observed at tilmicosin dosage >7.5 mg/kg. The PK/PD parameter of AUC/MIC (The area under the concentration–time curve divided by the minimal inhibitory concentration) correlated well with anti-mycoplasmal efficacy (R(2) = 0.92). The ratios of AUC/MIC for 1 log(10) and 3 log(10) colony-forming units [CFU]/lung reductions were 300.02 and 6,950.15 h, respectively. These findings indicated that tilmicosin may be therapeutically effective in chickens to treat M. gallisepticum lung infections if administered at a dose of 9.12 mg/kg.
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spelling pubmed-95570782022-10-14 Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens Zhang, Nan Zhou, Minghu Yan, Xiu Liu, Jinxin Yuan, Sheng Yang, Hong Ding, Huanzhong Zhang, Dexian Bai, Yinshan Front Vet Sci Veterinary Science Mycoplasma gallisepticum (M. gallisepticum) is a primary respiratory pathogen of poultry and causes significant economic losses to the poultry industry. There were no reported articles concerning the Pharmacokinetics/Pharmacodynamics (PK/PD) interactions of tilmicosin against M. gallisepticum in vivo. In the current study, we established an in vivo M. gallisepticum infection model and tilmicosin was administered orally to the M. gallisepticum-infected chickens by different dosage regimens. The concentration of tilmicosin in lung tissue was determined by high-pressure liquid chromatography/tandem mass spectrometry (HPLC–MS/MS), besides the counting of the viable colony of M. gallisepticum in lung tissue was also monitored dynamically to appraise the PK/PD interactions of tilmicosin against M. gallisepticum. We found that anti-mycoplasmal activity was concentration-dependent and mycoplasmacidal activity was observed at tilmicosin dosage >7.5 mg/kg. The PK/PD parameter of AUC/MIC (The area under the concentration–time curve divided by the minimal inhibitory concentration) correlated well with anti-mycoplasmal efficacy (R(2) = 0.92). The ratios of AUC/MIC for 1 log(10) and 3 log(10) colony-forming units [CFU]/lung reductions were 300.02 and 6,950.15 h, respectively. These findings indicated that tilmicosin may be therapeutically effective in chickens to treat M. gallisepticum lung infections if administered at a dose of 9.12 mg/kg. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9557078/ /pubmed/36246335 http://dx.doi.org/10.3389/fvets.2022.952599 Text en Copyright © 2022 Zhang, Zhou, Yan, Liu, Yuan, Yang, Ding, Zhang and Bai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhang, Nan
Zhou, Minghu
Yan, Xiu
Liu, Jinxin
Yuan, Sheng
Yang, Hong
Ding, Huanzhong
Zhang, Dexian
Bai, Yinshan
Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens
title Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens
title_full Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens
title_fullStr Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens
title_full_unstemmed Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens
title_short Pharmacokinetic and Pharmacodynamic integration of tilmicosin against Mycoplasma gallisepticum in the target infection site in chickens
title_sort pharmacokinetic and pharmacodynamic integration of tilmicosin against mycoplasma gallisepticum in the target infection site in chickens
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557078/
https://www.ncbi.nlm.nih.gov/pubmed/36246335
http://dx.doi.org/10.3389/fvets.2022.952599
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