Cargando…

Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice

Although diabetic cognitive impairment is one of the most common complications of type 2 diabetes mellitus (T2DM), optimized therapeutic strategies are not available yet. Astragalosides IV (AS-IV) is a traditional Chinese medicine possessing diverse pharmacological properties including anti-inflamma...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yaxuan, Yuan, Yuan, Zhang, Jiawei, Zhao, Yao, Zhang, Yueqi, Fu, Jianliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557080/
https://www.ncbi.nlm.nih.gov/pubmed/36247985
http://dx.doi.org/10.3389/fnagi.2022.1004557
_version_ 1784807222425944064
author Zhang, Yaxuan
Yuan, Yuan
Zhang, Jiawei
Zhao, Yao
Zhang, Yueqi
Fu, Jianliang
author_facet Zhang, Yaxuan
Yuan, Yuan
Zhang, Jiawei
Zhao, Yao
Zhang, Yueqi
Fu, Jianliang
author_sort Zhang, Yaxuan
collection PubMed
description Although diabetic cognitive impairment is one of the most common complications of type 2 diabetes mellitus (T2DM), optimized therapeutic strategies are not available yet. Astragalosides IV (AS-IV) is a traditional Chinese medicine possessing diverse pharmacological properties including anti-inflammatory and antioxidant effects. However, the effects of AS-IV on diabetes-related cognitive impairment and its precise mechanisms remain largely unknown. T2DM mice, induced by a high-fat diet (HFD) and an intraperitoneal injection of low-dose streptozotocin (STZ) were administrated with AS-IV every other day for eight consecutive weeks. Learning and memory abilities were assessed subsequently using the Ymaze test and the anxious behavior was evaluated using an open field test. Then, the morphology and number of neurons and microglia were observed by HE staining or immunohistochemistry. Oxidative stress biomarkers and pro-inflammatory cytokines were determined using relevant kits. In addition, the expression levels of Nrf2, Keap1, HO-1, and NQO1 were determined by Western blot analyses. The results indicated that AS-IV administration significantly improved neuronal damage and cognitive deficit in T2DM mice. Meanwhile, oxidative stress and neuroinflammation were also ameliorated in T2DM mice, which might be attributed to the regulation of Nrf2/Keap1/HO-1/NQO1 pathway in T2DM mice. Taken together, these data suggested that AS-IV ameliorates cognitive impairment in T2DM mice by attenuating oxidative stress and neuroinflammation, possibly through modulating the Nrf2/Keap1/HO1/NQO1 pathway.
format Online
Article
Text
id pubmed-9557080
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95570802022-10-14 Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice Zhang, Yaxuan Yuan, Yuan Zhang, Jiawei Zhao, Yao Zhang, Yueqi Fu, Jianliang Front Aging Neurosci Aging Neuroscience Although diabetic cognitive impairment is one of the most common complications of type 2 diabetes mellitus (T2DM), optimized therapeutic strategies are not available yet. Astragalosides IV (AS-IV) is a traditional Chinese medicine possessing diverse pharmacological properties including anti-inflammatory and antioxidant effects. However, the effects of AS-IV on diabetes-related cognitive impairment and its precise mechanisms remain largely unknown. T2DM mice, induced by a high-fat diet (HFD) and an intraperitoneal injection of low-dose streptozotocin (STZ) were administrated with AS-IV every other day for eight consecutive weeks. Learning and memory abilities were assessed subsequently using the Ymaze test and the anxious behavior was evaluated using an open field test. Then, the morphology and number of neurons and microglia were observed by HE staining or immunohistochemistry. Oxidative stress biomarkers and pro-inflammatory cytokines were determined using relevant kits. In addition, the expression levels of Nrf2, Keap1, HO-1, and NQO1 were determined by Western blot analyses. The results indicated that AS-IV administration significantly improved neuronal damage and cognitive deficit in T2DM mice. Meanwhile, oxidative stress and neuroinflammation were also ameliorated in T2DM mice, which might be attributed to the regulation of Nrf2/Keap1/HO-1/NQO1 pathway in T2DM mice. Taken together, these data suggested that AS-IV ameliorates cognitive impairment in T2DM mice by attenuating oxidative stress and neuroinflammation, possibly through modulating the Nrf2/Keap1/HO1/NQO1 pathway. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9557080/ /pubmed/36247985 http://dx.doi.org/10.3389/fnagi.2022.1004557 Text en Copyright © 2022 Zhang, Yuan, Zhang, Zhao, Zhang and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging Neuroscience
Zhang, Yaxuan
Yuan, Yuan
Zhang, Jiawei
Zhao, Yao
Zhang, Yueqi
Fu, Jianliang
Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
title Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
title_full Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
title_fullStr Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
title_full_unstemmed Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
title_short Astragaloside IV supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
title_sort astragaloside iv supplementation attenuates cognitive impairment by inhibiting neuroinflammation and oxidative stress in type 2 diabetic mice
topic Aging Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557080/
https://www.ncbi.nlm.nih.gov/pubmed/36247985
http://dx.doi.org/10.3389/fnagi.2022.1004557
work_keys_str_mv AT zhangyaxuan astragalosideivsupplementationattenuatescognitiveimpairmentbyinhibitingneuroinflammationandoxidativestressintype2diabeticmice
AT yuanyuan astragalosideivsupplementationattenuatescognitiveimpairmentbyinhibitingneuroinflammationandoxidativestressintype2diabeticmice
AT zhangjiawei astragalosideivsupplementationattenuatescognitiveimpairmentbyinhibitingneuroinflammationandoxidativestressintype2diabeticmice
AT zhaoyao astragalosideivsupplementationattenuatescognitiveimpairmentbyinhibitingneuroinflammationandoxidativestressintype2diabeticmice
AT zhangyueqi astragalosideivsupplementationattenuatescognitiveimpairmentbyinhibitingneuroinflammationandoxidativestressintype2diabeticmice
AT fujianliang astragalosideivsupplementationattenuatescognitiveimpairmentbyinhibitingneuroinflammationandoxidativestressintype2diabeticmice