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Actin capping protein regulates postsynaptic spine development through CPI-motif interactions

Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable spines containing an expanded head. Remodeling of the actin...

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Autores principales: Myers, Kenneth R., Fan, Yanjie, McConnell, Patrick, Cooper, John A., Zheng, James Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557104/
https://www.ncbi.nlm.nih.gov/pubmed/36245917
http://dx.doi.org/10.3389/fnmol.2022.1020949
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author Myers, Kenneth R.
Fan, Yanjie
McConnell, Patrick
Cooper, John A.
Zheng, James Q.
author_facet Myers, Kenneth R.
Fan, Yanjie
McConnell, Patrick
Cooper, John A.
Zheng, James Q.
author_sort Myers, Kenneth R.
collection PubMed
description Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable spines containing an expanded head. Remodeling of the actin cytoskeleton plays a key role in the formation and modification of spine morphology, however many of the underlying regulatory mechanisms remain unclear. Capping protein (CP) is a major actin regulating protein that caps the barbed ends of actin filaments, and promotes the formation of dense branched actin networks. Knockdown of CP impairs the formation of mature spines, leading to an increase in the number of filopodia-like protrusions and defects in synaptic transmission. Here, we show that CP promotes the stabilization of dendritic protrusions, leading to the formation of stable mature spines. However, the localization and function of CP in dendritic spines requires interactions with proteins containing a capping protein interaction (CPI) motif. We found that the CPI motif-containing protein Twinfilin-1 (Twf1) also localizes to spines where it plays a role in CP spine enrichment. The knockdown of Twf1 leads to an increase in the density of filopodia-like protrusions and a decrease in the stability of dendritic protrusions, similar to CP knockdown. Finally, we show that CP directly interacts with Shank and regulates its spine accumulation. These results suggest that spatiotemporal regulation of CP in spines not only controls the actin dynamics underlying the formation of stable postsynaptic spine structures, but also plays an important role in the assembly of the postsynaptic apparatus underlying synaptic function.
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spelling pubmed-95571042022-10-14 Actin capping protein regulates postsynaptic spine development through CPI-motif interactions Myers, Kenneth R. Fan, Yanjie McConnell, Patrick Cooper, John A. Zheng, James Q. Front Mol Neurosci Neuroscience Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable spines containing an expanded head. Remodeling of the actin cytoskeleton plays a key role in the formation and modification of spine morphology, however many of the underlying regulatory mechanisms remain unclear. Capping protein (CP) is a major actin regulating protein that caps the barbed ends of actin filaments, and promotes the formation of dense branched actin networks. Knockdown of CP impairs the formation of mature spines, leading to an increase in the number of filopodia-like protrusions and defects in synaptic transmission. Here, we show that CP promotes the stabilization of dendritic protrusions, leading to the formation of stable mature spines. However, the localization and function of CP in dendritic spines requires interactions with proteins containing a capping protein interaction (CPI) motif. We found that the CPI motif-containing protein Twinfilin-1 (Twf1) also localizes to spines where it plays a role in CP spine enrichment. The knockdown of Twf1 leads to an increase in the density of filopodia-like protrusions and a decrease in the stability of dendritic protrusions, similar to CP knockdown. Finally, we show that CP directly interacts with Shank and regulates its spine accumulation. These results suggest that spatiotemporal regulation of CP in spines not only controls the actin dynamics underlying the formation of stable postsynaptic spine structures, but also plays an important role in the assembly of the postsynaptic apparatus underlying synaptic function. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9557104/ /pubmed/36245917 http://dx.doi.org/10.3389/fnmol.2022.1020949 Text en Copyright © 2022 Myers, Fan, McConnell, Cooper and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Myers, Kenneth R.
Fan, Yanjie
McConnell, Patrick
Cooper, John A.
Zheng, James Q.
Actin capping protein regulates postsynaptic spine development through CPI-motif interactions
title Actin capping protein regulates postsynaptic spine development through CPI-motif interactions
title_full Actin capping protein regulates postsynaptic spine development through CPI-motif interactions
title_fullStr Actin capping protein regulates postsynaptic spine development through CPI-motif interactions
title_full_unstemmed Actin capping protein regulates postsynaptic spine development through CPI-motif interactions
title_short Actin capping protein regulates postsynaptic spine development through CPI-motif interactions
title_sort actin capping protein regulates postsynaptic spine development through cpi-motif interactions
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557104/
https://www.ncbi.nlm.nih.gov/pubmed/36245917
http://dx.doi.org/10.3389/fnmol.2022.1020949
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