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Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells

Tumor immunotherapy has become one of the most promising approaches to tumor treatment. This study aimed to screen genes involved in the response of clear cell renal cell carcinoma (ccRCC) to immunotherapy and analyze their function. Based on the Gene Expression Omnibus and The Cancer Genome Atlas d...

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Autores principales: Fang, Guodong, Wang, Xudan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557226/
https://www.ncbi.nlm.nih.gov/pubmed/36247009
http://dx.doi.org/10.3389/fcell.2022.892616
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author Fang, Guodong
Wang, Xudan
author_facet Fang, Guodong
Wang, Xudan
author_sort Fang, Guodong
collection PubMed
description Tumor immunotherapy has become one of the most promising approaches to tumor treatment. This study aimed to screen genes involved in the response of clear cell renal cell carcinoma (ccRCC) to immunotherapy and analyze their function. Based on the Gene Expression Omnibus and The Cancer Genome Atlas datasets, we screened out nine differentially expressed genes (TYROBP, APOC1, CSTA, LY96, LAPTM5, CD300A, ALOX5, C1QA, and C1QB) associated with clinical traits and prognosis. A risk signature constructed by these nine genes could predict the survival probability for patients at 1 year, 3 years, and 5 years. The immune checkpoint blockade response rate in the high-risk group was significantly higher than in the low-risk group (49.25% vs. 24.72%, p ≤ 0.001). The nine prognosis-related genes were negatively correlated with activated dendritic cells in the low-risk group but not in the high-risk group. qRT-PCR, immunohistochemistry, and immunofluorescence showed that the nine prognosis-related genes were associated with dendritic cell activity and the PD-1 positive staining rate. In conclusion, the nine prognosis-related genes have a high prognostic value. The patients in the high-risk group were more likely to benefit from immunotherapy, and the mechanism might be related to the release of dendritic cell-mediated immunosuppression.
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spelling pubmed-95572262022-10-14 Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells Fang, Guodong Wang, Xudan Front Cell Dev Biol Cell and Developmental Biology Tumor immunotherapy has become one of the most promising approaches to tumor treatment. This study aimed to screen genes involved in the response of clear cell renal cell carcinoma (ccRCC) to immunotherapy and analyze their function. Based on the Gene Expression Omnibus and The Cancer Genome Atlas datasets, we screened out nine differentially expressed genes (TYROBP, APOC1, CSTA, LY96, LAPTM5, CD300A, ALOX5, C1QA, and C1QB) associated with clinical traits and prognosis. A risk signature constructed by these nine genes could predict the survival probability for patients at 1 year, 3 years, and 5 years. The immune checkpoint blockade response rate in the high-risk group was significantly higher than in the low-risk group (49.25% vs. 24.72%, p ≤ 0.001). The nine prognosis-related genes were negatively correlated with activated dendritic cells in the low-risk group but not in the high-risk group. qRT-PCR, immunohistochemistry, and immunofluorescence showed that the nine prognosis-related genes were associated with dendritic cell activity and the PD-1 positive staining rate. In conclusion, the nine prognosis-related genes have a high prognostic value. The patients in the high-risk group were more likely to benefit from immunotherapy, and the mechanism might be related to the release of dendritic cell-mediated immunosuppression. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9557226/ /pubmed/36247009 http://dx.doi.org/10.3389/fcell.2022.892616 Text en Copyright © 2022 Fang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Fang, Guodong
Wang, Xudan
Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
title Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
title_full Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
title_fullStr Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
title_full_unstemmed Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
title_short Prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
title_sort prognosis-related genes participate in immunotherapy of renal clear cell carcinoma possibly by targeting dendritic cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557226/
https://www.ncbi.nlm.nih.gov/pubmed/36247009
http://dx.doi.org/10.3389/fcell.2022.892616
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