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Target-switch SELEX: Screening with alternating targets to generate aptamers to conserved terminal dipeptides

Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution. We describe here the design and selection steps for discovery of DNA aptamers with...

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Detalles Bibliográficos
Autores principales:	Cutts, Zachary William, Hong, Jessica M., Shao, Shirley, Tran, Alexander, Dimon, Michelle, Berndl, Marc, Wu, Diana, Pawlosky, Annalisa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557731/ https://www.ncbi.nlm.nih.gov/pubmed/36208449 http://dx.doi.org/10.1016/j.xpro.2022.101724

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