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Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean
Soybean [Glycine max (L.) Merri.] is one of the most valuable global crops. And vegetable soybean, as a special type of soybean, provides rich nutrition in people’s life. In order to investigate the gene expression networks and molecular regulatory mechanisms that regulate soybean seed oil and prote...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557928/ https://www.ncbi.nlm.nih.gov/pubmed/36247601 http://dx.doi.org/10.3389/fpls.2022.1012394 |
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author | Xu, Wenjing Wang, Qiong Zhang, Wei Zhang, Hongmei Liu, Xiaoqing Song, Qingxin Zhu, Yuelin Cui, Xiaoyan Chen, Xin Chen, Huatao |
author_facet | Xu, Wenjing Wang, Qiong Zhang, Wei Zhang, Hongmei Liu, Xiaoqing Song, Qingxin Zhu, Yuelin Cui, Xiaoyan Chen, Xin Chen, Huatao |
author_sort | Xu, Wenjing |
collection | PubMed |
description | Soybean [Glycine max (L.) Merri.] is one of the most valuable global crops. And vegetable soybean, as a special type of soybean, provides rich nutrition in people’s life. In order to investigate the gene expression networks and molecular regulatory mechanisms that regulate soybean seed oil and protein contents during seed development, we performed transcriptomic and metabolomic analyses of soybean seeds during development in two soybean varieties that differ in protein and oil contents. We identified a total of 41,036 genes and 392 metabolites, of which 12,712 DEGs and 315 DAMs were identified. Analysis of KEGG enrichment demonstrated that DEGs were primarily enriched in phenylpropanoid biosynthesis, glycerolipid metabolism, carbon metabolism, plant hormone signal transduction, linoleic acid metabolism, and the biosynthesis of amino acids and secondary metabolites. K-means analysis divided the DEGs into 12 distinct clusters. We identified candidate gene sets that regulate the biosynthesis of protein and oil in soybean seeds, and present potential regulatory patterns that high seed-protein varieties may be more sensitive to desiccation, show earlier photomorphogenesis and delayed leaf senescence, and thus accumulate higher protein contents than high-oil varieties. |
format | Online Article Text |
id | pubmed-9557928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95579282022-10-14 Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean Xu, Wenjing Wang, Qiong Zhang, Wei Zhang, Hongmei Liu, Xiaoqing Song, Qingxin Zhu, Yuelin Cui, Xiaoyan Chen, Xin Chen, Huatao Front Plant Sci Plant Science Soybean [Glycine max (L.) Merri.] is one of the most valuable global crops. And vegetable soybean, as a special type of soybean, provides rich nutrition in people’s life. In order to investigate the gene expression networks and molecular regulatory mechanisms that regulate soybean seed oil and protein contents during seed development, we performed transcriptomic and metabolomic analyses of soybean seeds during development in two soybean varieties that differ in protein and oil contents. We identified a total of 41,036 genes and 392 metabolites, of which 12,712 DEGs and 315 DAMs were identified. Analysis of KEGG enrichment demonstrated that DEGs were primarily enriched in phenylpropanoid biosynthesis, glycerolipid metabolism, carbon metabolism, plant hormone signal transduction, linoleic acid metabolism, and the biosynthesis of amino acids and secondary metabolites. K-means analysis divided the DEGs into 12 distinct clusters. We identified candidate gene sets that regulate the biosynthesis of protein and oil in soybean seeds, and present potential regulatory patterns that high seed-protein varieties may be more sensitive to desiccation, show earlier photomorphogenesis and delayed leaf senescence, and thus accumulate higher protein contents than high-oil varieties. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9557928/ /pubmed/36247601 http://dx.doi.org/10.3389/fpls.2022.1012394 Text en Copyright © 2022 Xu, Wang, Zhang, Zhang, Liu, Song, Zhu, Cui, Chen and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xu, Wenjing Wang, Qiong Zhang, Wei Zhang, Hongmei Liu, Xiaoqing Song, Qingxin Zhu, Yuelin Cui, Xiaoyan Chen, Xin Chen, Huatao Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
title | Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
title_full | Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
title_fullStr | Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
title_full_unstemmed | Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
title_short | Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
title_sort | using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9557928/ https://www.ncbi.nlm.nih.gov/pubmed/36247601 http://dx.doi.org/10.3389/fpls.2022.1012394 |
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