Immediate protein expression from exogenous mRNAs in embryonic brain

mRNA vaccines for SARS-CoV-2 have been widely used and saving millions of people in the world. How efficiently proteins are produced from exogenous mRNAs in the embryonic brain, however, is less known. Here we show that protein expression occurs highly efficiently in neural stem cells, in a very nar...

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Detalles Bibliográficos
Autores principales: Naruse, Masae, Saito, Tetsuichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558027/
https://www.ncbi.nlm.nih.gov/pubmed/36229519
http://dx.doi.org/10.1038/s41598-022-21668-5
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author Naruse, Masae
Saito, Tetsuichiro
author_facet Naruse, Masae
Saito, Tetsuichiro
author_sort Naruse, Masae
collection PubMed
description mRNA vaccines for SARS-CoV-2 have been widely used and saving millions of people in the world. How efficiently proteins are produced from exogenous mRNAs in the embryonic brain, however, is less known. Here we show that protein expression occurs highly efficiently in neural stem cells, in a very narrow time window after mRNA electroporation in the embryonic mouse brain, where plasmids have been successfully transfected. Protein expression is detected 1 h and 12 h after the electroporation of mRNAs and plasmids, respectively. The delivery of exogenous mRNAs may be useful for not only vaccines but also functional analysis in the brain.
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spelling pubmed-95580272022-10-13 Immediate protein expression from exogenous mRNAs in embryonic brain Naruse, Masae Saito, Tetsuichiro Sci Rep Article mRNA vaccines for SARS-CoV-2 have been widely used and saving millions of people in the world. How efficiently proteins are produced from exogenous mRNAs in the embryonic brain, however, is less known. Here we show that protein expression occurs highly efficiently in neural stem cells, in a very narrow time window after mRNA electroporation in the embryonic mouse brain, where plasmids have been successfully transfected. Protein expression is detected 1 h and 12 h after the electroporation of mRNAs and plasmids, respectively. The delivery of exogenous mRNAs may be useful for not only vaccines but also functional analysis in the brain. Nature Publishing Group UK 2022-10-13 /pmc/articles/PMC9558027/ /pubmed/36229519 http://dx.doi.org/10.1038/s41598-022-21668-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Naruse, Masae
Saito, Tetsuichiro
Immediate protein expression from exogenous mRNAs in embryonic brain
title Immediate protein expression from exogenous mRNAs in embryonic brain
title_full Immediate protein expression from exogenous mRNAs in embryonic brain
title_fullStr Immediate protein expression from exogenous mRNAs in embryonic brain
title_full_unstemmed Immediate protein expression from exogenous mRNAs in embryonic brain
title_short Immediate protein expression from exogenous mRNAs in embryonic brain
title_sort immediate protein expression from exogenous mrnas in embryonic brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558027/
https://www.ncbi.nlm.nih.gov/pubmed/36229519
http://dx.doi.org/10.1038/s41598-022-21668-5
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