Quantification and characterization of mouse and human tissue-resident microbiota by qPCR and 16S sequencing

The tissue-resident microbiota is an integral component of multiple tumor types, but it remains challenging to characterize its abundance and composition due to its low biomass. Here, we describe an optimized protocol for quantification and profiling of tissue-resident microbiota. The major optimize...

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Detalles Bibliográficos
Autores principales: Yao, Bingqing, Dong, Tingting, Fu, Aikun, Li, Hang, Jiang, Chuhan, Li, Nan, Cai, Shang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558047/
https://www.ncbi.nlm.nih.gov/pubmed/36209427
http://dx.doi.org/10.1016/j.xpro.2022.101765

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558047/
https://www.ncbi.nlm.nih.gov/pubmed/36209427
http://dx.doi.org/10.1016/j.xpro.2022.101765

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