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Quantification and characterization of mouse and human tissue-resident microbiota by qPCR and 16S sequencing
The tissue-resident microbiota is an integral component of multiple tumor types, but it remains challenging to characterize its abundance and composition due to its low biomass. Here, we describe an optimized protocol for quantification and profiling of tissue-resident microbiota. The major optimize...
Autores principales: | Yao, Bingqing, Dong, Tingting, Fu, Aikun, Li, Hang, Jiang, Chuhan, Li, Nan, Cai, Shang |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558047/ https://www.ncbi.nlm.nih.gov/pubmed/36209427 http://dx.doi.org/10.1016/j.xpro.2022.101765 |
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