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Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay

BACKGROUND: Two antigenically and genetically distinct lineages of influenza B viruses (B/Victoria and B/Yamagata) have been co-circulating worldwide since 2002. Virological surveillance is essential to differentiate between both lineages with a view to the annual updating of the B component for the...

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Autores principales: da Silva, Daniela Bernardes Borges, de Oliveira Santos, Katia Corrêa, Benega, Margarete Aparecida, de Paiva, Terezinha Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558098/
https://www.ncbi.nlm.nih.gov/pubmed/36246545
http://dx.doi.org/10.1016/j.jvacx.2022.100220
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author da Silva, Daniela Bernardes Borges
de Oliveira Santos, Katia Corrêa
Benega, Margarete Aparecida
de Paiva, Terezinha Maria
author_facet da Silva, Daniela Bernardes Borges
de Oliveira Santos, Katia Corrêa
Benega, Margarete Aparecida
de Paiva, Terezinha Maria
author_sort da Silva, Daniela Bernardes Borges
collection PubMed
description BACKGROUND: Two antigenically and genetically distinct lineages of influenza B viruses (B/Victoria and B/Yamagata) have been co-circulating worldwide since 2002. Virological surveillance is essential to differentiate between both lineages with a view to the annual updating of the B component for the trivalent or quadrivalent influenza vaccine composition. METHODS: The samples analyzed in the present study were collected by influenza sentinel units located in the Southeast, Midwest, North, and Northeast regions of Brazil, part of the National Influenza Virus Surveillance Network, coordinated by the Ministry of Health of Brazil. A total of 870 influenza B positive samples by reverse transcription real – time polymerase chain reaction (RT-qPCR), collected during 2014, 2015, and 2016 influenza seasons, were submitted to the influenza B lineage genotyping panel for characterization as B/Yamagata or Victoria lineages using RT-qPCR. RESULTS: Of the 197 samples analyzed in 2014, a total of 160 (81 %) corresponded to the B/Yamagata lineage, 19 (10 %) to the B/Victoria lineage, and 18 (9 %) to indeterminate lineages. Of the 190 samples analyzed in 2015, a total of 124 (65 %) corresponded to the B/Yamagata lineage; 55 (29 %) to the B/Victoria lineage, whereas 11 (6 %) were of indeterminate lineages. Of the 483 samples analyzed in 2016, a total of 297 (62 %) corresponded to the B /Victoria lineage; 174 (36 %) to the B/Yamagata lineage and 12 (2 %) to indeterminate lineages. This cross-sectional study revealed influenza B virus (IBV) infection in all age groups, and among them, the highest prevalence was observed in individuals between 11 and 49 years of age Our findings demonstrate the match between influenza B virus lineages recommended by the World Health Organization (WHO) for the trivalent vaccine composition to be used in the Southern Hemisphere (SH) and the predominant circulating viruses during the 2014, 2015, and 2016 seasons.
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spelling pubmed-95580982022-10-14 Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay da Silva, Daniela Bernardes Borges de Oliveira Santos, Katia Corrêa Benega, Margarete Aparecida de Paiva, Terezinha Maria Vaccine X Regular paper BACKGROUND: Two antigenically and genetically distinct lineages of influenza B viruses (B/Victoria and B/Yamagata) have been co-circulating worldwide since 2002. Virological surveillance is essential to differentiate between both lineages with a view to the annual updating of the B component for the trivalent or quadrivalent influenza vaccine composition. METHODS: The samples analyzed in the present study were collected by influenza sentinel units located in the Southeast, Midwest, North, and Northeast regions of Brazil, part of the National Influenza Virus Surveillance Network, coordinated by the Ministry of Health of Brazil. A total of 870 influenza B positive samples by reverse transcription real – time polymerase chain reaction (RT-qPCR), collected during 2014, 2015, and 2016 influenza seasons, were submitted to the influenza B lineage genotyping panel for characterization as B/Yamagata or Victoria lineages using RT-qPCR. RESULTS: Of the 197 samples analyzed in 2014, a total of 160 (81 %) corresponded to the B/Yamagata lineage, 19 (10 %) to the B/Victoria lineage, and 18 (9 %) to indeterminate lineages. Of the 190 samples analyzed in 2015, a total of 124 (65 %) corresponded to the B/Yamagata lineage; 55 (29 %) to the B/Victoria lineage, whereas 11 (6 %) were of indeterminate lineages. Of the 483 samples analyzed in 2016, a total of 297 (62 %) corresponded to the B /Victoria lineage; 174 (36 %) to the B/Yamagata lineage and 12 (2 %) to indeterminate lineages. This cross-sectional study revealed influenza B virus (IBV) infection in all age groups, and among them, the highest prevalence was observed in individuals between 11 and 49 years of age Our findings demonstrate the match between influenza B virus lineages recommended by the World Health Organization (WHO) for the trivalent vaccine composition to be used in the Southern Hemisphere (SH) and the predominant circulating viruses during the 2014, 2015, and 2016 seasons. Elsevier 2022-09-30 /pmc/articles/PMC9558098/ /pubmed/36246545 http://dx.doi.org/10.1016/j.jvacx.2022.100220 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular paper
da Silva, Daniela Bernardes Borges
de Oliveira Santos, Katia Corrêa
Benega, Margarete Aparecida
de Paiva, Terezinha Maria
Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay
title Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay
title_full Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay
title_fullStr Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay
title_full_unstemmed Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay
title_short Differentiation of influenza B lineages circulating in different regions of Brazil, 2014–2016, using molecular assay
title_sort differentiation of influenza b lineages circulating in different regions of brazil, 2014–2016, using molecular assay
topic Regular paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558098/
https://www.ncbi.nlm.nih.gov/pubmed/36246545
http://dx.doi.org/10.1016/j.jvacx.2022.100220
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