Cargando…

Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken

Skeletal muscle, comprising approximately 40% of body mass, is a highly complex and heterogeneous tissue serving a multitude of functions in the organism. Non-coding RNAs (ncRNAs) are known to participate in skeletal muscle development as critical regulators. However, the regulatory mechanisms of nc...

Descripción completa

Detalles Bibliográficos
Autores principales: Pan, Zegun, Yang, Chaowu, Zhao, Ruipeng, Jiang, Xiaosong, Yu, Chunli, Li, Zhixiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558166/
https://www.ncbi.nlm.nih.gov/pubmed/36246144
http://dx.doi.org/10.3389/fphys.2022.969854
_version_ 1784807387051327488
author Pan, Zegun
Yang, Chaowu
Zhao, Ruipeng
Jiang, Xiaosong
Yu, Chunli
Li, Zhixiong
author_facet Pan, Zegun
Yang, Chaowu
Zhao, Ruipeng
Jiang, Xiaosong
Yu, Chunli
Li, Zhixiong
author_sort Pan, Zegun
collection PubMed
description Skeletal muscle, comprising approximately 40% of body mass, is a highly complex and heterogeneous tissue serving a multitude of functions in the organism. Non-coding RNAs (ncRNAs) are known to participate in skeletal muscle development as critical regulators. However, the regulatory mechanisms of ncRNAs on chicken muscle traits are not well understood. In the present study, we collected the leg muscle from male embryos of Tibetan chicken at embryonic (E) 10 and E18 for RNA sequencing. A total of 6,583 differentially expressed mRNAs (DEMs) including 3,055 down-regulated and 3,528 up-regulated were identified in E18. We identified 695 differentially expressed lncRNAs (DELs) (187 down-regulated and 508 up-regulated) and 1,906 differentially expressed circRNAs (DECs) (1,224 down-regulated and 682 up-regulated) in E18. Among the 130 differentially expressed miRNAs (DEMIs), 59 were up-regulated and 71 were down-regulated in E18. Numerous DEMs and target genes for miRNAs/lncRNAs were significantly enriched in the muscle system process and cell cycle. We constructed a miRNA-gene-pathway network by considering target relationships between genes related to skeletal muscle development and miRNAs. A competing endogenous RNA (ceRNA) network was also constructed by integrating competing relationships between DEMs, DELs, and DECs. Several DELs and DECs were predicted to regulate the ADRA1B, ATP2A2, ATP2B1, CACNA1S, CACNB4, MYLK2, and ROCK2 genes. We discovered the crosstalk between the ncRNAs and their competing mRNAs, which provides insights into ceRNA function and mechanisms in the skeletal muscle development of chicken.
format Online
Article
Text
id pubmed-9558166
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95581662022-10-14 Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken Pan, Zegun Yang, Chaowu Zhao, Ruipeng Jiang, Xiaosong Yu, Chunli Li, Zhixiong Front Physiol Physiology Skeletal muscle, comprising approximately 40% of body mass, is a highly complex and heterogeneous tissue serving a multitude of functions in the organism. Non-coding RNAs (ncRNAs) are known to participate in skeletal muscle development as critical regulators. However, the regulatory mechanisms of ncRNAs on chicken muscle traits are not well understood. In the present study, we collected the leg muscle from male embryos of Tibetan chicken at embryonic (E) 10 and E18 for RNA sequencing. A total of 6,583 differentially expressed mRNAs (DEMs) including 3,055 down-regulated and 3,528 up-regulated were identified in E18. We identified 695 differentially expressed lncRNAs (DELs) (187 down-regulated and 508 up-regulated) and 1,906 differentially expressed circRNAs (DECs) (1,224 down-regulated and 682 up-regulated) in E18. Among the 130 differentially expressed miRNAs (DEMIs), 59 were up-regulated and 71 were down-regulated in E18. Numerous DEMs and target genes for miRNAs/lncRNAs were significantly enriched in the muscle system process and cell cycle. We constructed a miRNA-gene-pathway network by considering target relationships between genes related to skeletal muscle development and miRNAs. A competing endogenous RNA (ceRNA) network was also constructed by integrating competing relationships between DEMs, DELs, and DECs. Several DELs and DECs were predicted to regulate the ADRA1B, ATP2A2, ATP2B1, CACNA1S, CACNB4, MYLK2, and ROCK2 genes. We discovered the crosstalk between the ncRNAs and their competing mRNAs, which provides insights into ceRNA function and mechanisms in the skeletal muscle development of chicken. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9558166/ /pubmed/36246144 http://dx.doi.org/10.3389/fphys.2022.969854 Text en Copyright © 2022 Pan, Yang, Zhao, Jiang, Yu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Pan, Zegun
Yang, Chaowu
Zhao, Ruipeng
Jiang, Xiaosong
Yu, Chunli
Li, Zhixiong
Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken
title Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken
title_full Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken
title_fullStr Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken
title_full_unstemmed Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken
title_short Characterization of lncRNA/circRNA-miRNA-mRNA network to reveal potential functional ceRNAs in the skeletal muscle of chicken
title_sort characterization of lncrna/circrna-mirna-mrna network to reveal potential functional cernas in the skeletal muscle of chicken
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558166/
https://www.ncbi.nlm.nih.gov/pubmed/36246144
http://dx.doi.org/10.3389/fphys.2022.969854
work_keys_str_mv AT panzegun characterizationoflncrnacircrnamirnamrnanetworktorevealpotentialfunctionalcernasintheskeletalmuscleofchicken
AT yangchaowu characterizationoflncrnacircrnamirnamrnanetworktorevealpotentialfunctionalcernasintheskeletalmuscleofchicken
AT zhaoruipeng characterizationoflncrnacircrnamirnamrnanetworktorevealpotentialfunctionalcernasintheskeletalmuscleofchicken
AT jiangxiaosong characterizationoflncrnacircrnamirnamrnanetworktorevealpotentialfunctionalcernasintheskeletalmuscleofchicken
AT yuchunli characterizationoflncrnacircrnamirnamrnanetworktorevealpotentialfunctionalcernasintheskeletalmuscleofchicken
AT lizhixiong characterizationoflncrnacircrnamirnamrnanetworktorevealpotentialfunctionalcernasintheskeletalmuscleofchicken