Cargando…

Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums

Globally, healthcare-associated infections (HAI) are the most frequent adverse outcome in healthcare delivery. Although bacterial biofilms contribute significantly to the incidence of HAI, few studies have investigated the efficacy of common disinfectants against dry-surface biofilms (DSB). The obje...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaggar, Gurpreet K., Nkemngong, Carine A., Li, Xiaobao, Teska, Peter J., Oliver, Haley F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558353/
https://www.ncbi.nlm.nih.gov/pubmed/35275049
http://dx.doi.org/10.1099/mic.0.001140
_version_ 1784807425688207360
author Chaggar, Gurpreet K.
Nkemngong, Carine A.
Li, Xiaobao
Teska, Peter J.
Oliver, Haley F.
author_facet Chaggar, Gurpreet K.
Nkemngong, Carine A.
Li, Xiaobao
Teska, Peter J.
Oliver, Haley F.
author_sort Chaggar, Gurpreet K.
collection PubMed
description Globally, healthcare-associated infections (HAI) are the most frequent adverse outcome in healthcare delivery. Although bacterial biofilms contribute significantly to the incidence of HAI, few studies have investigated the efficacy of common disinfectants against dry-surface biofilms (DSB). The objective of this study was to evaluate the bactericidal efficacy of seven Environmental Protection Agency (EPA)-registered liquid disinfectants against DSB of Staphylococcus aureus and Pseudomonas aeruginosa . We hypothesized that overall, there will be significant differences among the bactericidal efficacies of tested disinfectants by product type and active ingredient class. We also hypothesized that depending on the species, higher bactericidal efficacies against DSB will be exhibited after 24 h of dehydration compared to 72 h. Wet-surface biofilms of S. aureus and P. aeruginosa were grown following EPA-MLB-SOP-MB-19 and dehydrated for 24 and 72 h to establish DSB. Seven EPA-registered disinfectants were tested against dehydrated DSB following EPA-MLB-SOP-MB-20. Overall, quaternary ammonium plus alcohol, sodium dichloro-s-triazinetrione and hydrogen peroxide products were more efficacious against DSB than quaternary ammoniums for both tested species. While there was no significant difference in the log(10) reductions between 24 and 72 h S . aureus biofilms, significantly higher log(10) reductions were observed when products were challenged with 24 h P . aeruginosa DSB compared to 72 h P . aeruginosa DSB. Species type, active ingredient class and dry time significantly impact disinfectant efficacy against DSB of S. aureus or P. aeruginosa .
format Online
Article
Text
id pubmed-9558353
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-95583532022-10-14 Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums Chaggar, Gurpreet K. Nkemngong, Carine A. Li, Xiaobao Teska, Peter J. Oliver, Haley F. Microbiology (Reading) Microbial Interactions and Communities Globally, healthcare-associated infections (HAI) are the most frequent adverse outcome in healthcare delivery. Although bacterial biofilms contribute significantly to the incidence of HAI, few studies have investigated the efficacy of common disinfectants against dry-surface biofilms (DSB). The objective of this study was to evaluate the bactericidal efficacy of seven Environmental Protection Agency (EPA)-registered liquid disinfectants against DSB of Staphylococcus aureus and Pseudomonas aeruginosa . We hypothesized that overall, there will be significant differences among the bactericidal efficacies of tested disinfectants by product type and active ingredient class. We also hypothesized that depending on the species, higher bactericidal efficacies against DSB will be exhibited after 24 h of dehydration compared to 72 h. Wet-surface biofilms of S. aureus and P. aeruginosa were grown following EPA-MLB-SOP-MB-19 and dehydrated for 24 and 72 h to establish DSB. Seven EPA-registered disinfectants were tested against dehydrated DSB following EPA-MLB-SOP-MB-20. Overall, quaternary ammonium plus alcohol, sodium dichloro-s-triazinetrione and hydrogen peroxide products were more efficacious against DSB than quaternary ammoniums for both tested species. While there was no significant difference in the log(10) reductions between 24 and 72 h S . aureus biofilms, significantly higher log(10) reductions were observed when products were challenged with 24 h P . aeruginosa DSB compared to 72 h P . aeruginosa DSB. Species type, active ingredient class and dry time significantly impact disinfectant efficacy against DSB of S. aureus or P. aeruginosa . Microbiology Society 2022-03-11 /pmc/articles/PMC9558353/ /pubmed/35275049 http://dx.doi.org/10.1099/mic.0.001140 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Microbial Interactions and Communities
Chaggar, Gurpreet K.
Nkemngong, Carine A.
Li, Xiaobao
Teska, Peter J.
Oliver, Haley F.
Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums
title Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums
title_full Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums
title_fullStr Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums
title_full_unstemmed Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums
title_short Hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of Staphylococcus aureus and Pseudomonas aeruginosa more than quaternary ammoniums
title_sort hydrogen peroxide, sodium dichloro-s-triazinetriones and quaternary alcohols significantly inactivate the dry-surface biofilms of staphylococcus aureus and pseudomonas aeruginosa more than quaternary ammoniums
topic Microbial Interactions and Communities
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9558353/
https://www.ncbi.nlm.nih.gov/pubmed/35275049
http://dx.doi.org/10.1099/mic.0.001140
work_keys_str_mv AT chaggargurpreetk hydrogenperoxidesodiumdichlorostriazinetrionesandquaternaryalcoholssignificantlyinactivatethedrysurfacebiofilmsofstaphylococcusaureusandpseudomonasaeruginosamorethanquaternaryammoniums
AT nkemngongcarinea hydrogenperoxidesodiumdichlorostriazinetrionesandquaternaryalcoholssignificantlyinactivatethedrysurfacebiofilmsofstaphylococcusaureusandpseudomonasaeruginosamorethanquaternaryammoniums
AT lixiaobao hydrogenperoxidesodiumdichlorostriazinetrionesandquaternaryalcoholssignificantlyinactivatethedrysurfacebiofilmsofstaphylococcusaureusandpseudomonasaeruginosamorethanquaternaryammoniums
AT teskapeterj hydrogenperoxidesodiumdichlorostriazinetrionesandquaternaryalcoholssignificantlyinactivatethedrysurfacebiofilmsofstaphylococcusaureusandpseudomonasaeruginosamorethanquaternaryammoniums
AT oliverhaleyf hydrogenperoxidesodiumdichlorostriazinetrionesandquaternaryalcoholssignificantlyinactivatethedrysurfacebiofilmsofstaphylococcusaureusandpseudomonasaeruginosamorethanquaternaryammoniums